秦岭野生宜昌百合的组织培养研究

为了有效地保存、扩繁秦岭野生宜昌百合资源,以秦岭野生宜昌百合为材料,研究了鳞片和叶片不同部位,以及不同激素配比对不定芽诱导、增殖及生根的影响。结果表明,鳞片诱导不定芽的适宜培养基为MS+2.0mg/L 6-BA+0.05 mg/L NAA,鳞片分化不定芽的能力为基部>中部>上部,三者之间差异极显著;叶片诱导不定芽的适宜培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.0 mg/L 2,4-D,叶片不定芽的诱导率为基部高,中部和尖部低,且基部与中部和尖部差异极显著;不定芽增殖的适宜培养基为MS+1.0 mg/L 6-BA+0.05 mg/L NAA;适宜生根的培养基为1/2MS+0.4 mg/L NAA+1.0 g/L活性炭(AC)。以上结果表明,鳞片和叶片分化不定芽存在明显的位置效应,6-BA和NAA配合对不定芽增殖有良好的效果,NAA和AC配合适宜生根。

Tissue culture of wild 'Qinling Yichang' lily

With wild'Qinling Yichang'lily as materials,effects of parts of scales and leaves on inducing adventitious buds,and effects of hormone combination on inducing adventitious buds,propagating buds and rooting were studied in order to presevve and propagate the wildlily resources.The results showed that the appropriate medium of the adventitious buds induced directly from scales was MS 2.0 mg/L 6-BA 0.05 mg/L NAA.The ability of scales inducing adventitious buds was down>middle>upper;the suitable medium of the buds induced from leaves was MS 1.0 mg/L 6-BA 0.1 mg/L NAA 2.0 mg/L 2,4-D.The induction rates of adventitious bud were down was high,middle and upper were low;the optimum proliferation medium for adventitious buds was MS 1.0 mg/L 6-BA 0.05 mg/L NAA.The appropriate medium for rooting was 1/2MS 0.4 mg/L NAA 1.0 g/L AC.The above study showed that there was obvious rule about inducing adventitious buds with different parts of scales and leaves,the combination of 6-BA and NAA had good effects on propagating buds,and the combination of NAA and AC was suitable for rooting.