| 简并引物的程序化设计与荔枝HMGR基因片段的克隆 |
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| 引用本文： | 夏瑞,陆旺金,李建国,杜娟. 简并引物的程序化设计与荔枝HMGR基因片段的克隆[J]. 果树学报, 2006, 23(6): 903-906 |
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| 作者姓名： | 夏瑞 陆旺金 李建国 杜娟 |
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| 作者单位： | 1. 华南农业大学中国荔枝研究中心,广州,510642
2. 广东省果蔬保鲜重点实验室,广州,510642 |
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| 摘 要： | 主要介绍了利用NCBI、DDBJ、Blockmaker、CodeHop和Oligo6.0等数据库和生物软件进行简并引物设计的方法。利用设计的6对简并引物进行RT-PCR,从荔枝(LitchichinensisSonn.)果实中克隆到2个长度分别为510bp和582bp的cDNA片段。通过Blastx检索Genbank进行同源性比对后,发现2片段都与其它植物的HMGR基因具有较高的氨基酸序列同源性。研究表明,该程序化设计的简并引物可信性强,阳性率高,能迅速得到满意结果。
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| 关 键 词： | 荔枝 间并引物 程序化设计 HMGR基因 克隆 |
| 文章编号： | 1009-9980（2006）05-903-04 |
| 收稿时间： | 2006-05-24 |
| 修稿时间： | 2006-09-08 |
Programming design of degenerate primers and the cloning of litchi HMGR gene fragments |
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| XIA Rui,LU Wang-jin,LI Jian-guo,DU Juan. Programming design of degenerate primers and the cloning of litchi HMGR gene fragments[J]. Journal of Fruit Science, 2006, 23(6): 903-906 |
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| Authors: | XIA Rui LU Wang-jin LI Jian-guo DU Juan |
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| Affiliation: | 1China Litchi Research Center, South China Agricultural University, Guangzhou, Guangdong 510642 China; 2Guangdong Provincial Key Lab of Post-harvest Science of Fruits and Vegetables, Guangzhou, Guangdong 510642 China |
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| Abstract: | This paper aims to introduce the method of designing degenerate primers by using some internet databases and biological softwares such as NCBI, DDBJ, Blockmaker, CodeHop and Oligo 6.0. Using the designed 6 pairs of primers to carry out RT-PCR, 2 cDNA fragments with the length of 510 bp and 582 bp respectively were cloned from the litchi(Litchi chinensis Sonn.)young fruit. These 2 fragments were compared in the Genbank by blastx, and the results indicated that the 2 fragments had considerable identities with the HMGR genes of other plants. It is considered that the degenerate primers obtained from this programming design are practicable, and also get satisfied results and high positive rates. |
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| Keywords: | Litchi Degenerate primers Programming design HMGR gene Clone |
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