辽宁绒山羊BMP-2基因的克隆及序列比较分析

根据GenBank上绵羊的BMP-2基因序列设计特异性引物，以辽宁绒山羊基因组DNA为模板，利用聚合酶链式反应，成功克隆了常年长绒型和季节长绒型辽宁绒山羊BMP-2部分基因片段，丰富了绒山羊BMP-2基因序列。经与绵羊、牛、鼠、猪和人的BMP-2基因进行的比对结果表明，季节与常年长绒型辽宁绒山羊的BMP-2基因同源片段的同源性达到99．7％，二者与绵羊同源性为98．2％和98．4％；与牛同源性为98．2％和97．9％；与鼠同源性为86．3％和86％；与人同源性为88．1％和88．1％。结果表明，辽宁绒山羊BMP-2基因部分核苷酸序列与其他哺乳动物同源性很高，与绵羊、牛的同源性高达97％以上，这与它们的种属关系相近一致。与人、鼠的同源性也在86％以上，说明BMP-2基因在不同物种之间具有较高的保守性。

Cloning and Sequence Analyzing of B MP-2 Gene from Liaoning Cashmere Goats

According to the BMP-2 gene sequence of sheep on the GenBank, specific primers were designed and perennial and seasonal long-staple Cashmere goat BMP type-2 partial gene fragments were successfully cloned from the Liaoning cashmere goat genomic DNA, using the polymerase chain reaction. With the sheep, cat- tie, rats, pigs and human BMP-2 gene than on the results show that seasonal and perennial long-staple type of Liaoning cashmere goats homologous fragment of BMP-2 homology 99.7%, respeciively homology with the sheep was 98.2% and 98.4%; and bovine homology was 98.2% and 97.9%; and rat homolgy 86.3% and 86%; and human homology was 88.1% and 88.1%. The results showed that BMP-2 in Liaoning Cashmere goat gene partial nucleotide sequence homology with other mammals, which is similar to their relationship of species, and BMP-2 gene were conserved in different species.