Protectivity conferred by immunization with intranasal recombinant outer
membrane protein H from Pasteurella multocida serovar A:1 in
chickens |
| |
| Authors: | Arunee THANASARASAKULPONG Pichayanut POOLPERM Pallop TANKAEW Takuo SAWADA Nattawooti STHITMATEE |
| |
| Institution: | 1)Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;2)Faculty of Pharmacy, Payap University, Chiang Mai 50000, Thailand;3)Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan |
| |
| Abstract: | Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine
candidate. The present study was aimed at developing rOmpH formulations for intranasal
administration. The rOmpH was purified and formulated with either Escherichia
coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant.
Antibody responses in chickens intranasally immunized with rOmpH in combination with 2
different adjuvants were significantly increased (P<0.05) post
immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB
elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH
formulated with ODN elicited protection better than that formulated with LTB. Therefore,
the vaccines formulations in the present study can be considered new intranasal vaccine
formulations for fowl cholera in chickens. |
| |
| Keywords: | intranasal vaccine Pasteurella multocida recombinant outer membrane protein H |
|
|
