Isolation and characterization of canine tumor endothelial
cells |
| |
| Authors: | Yusuke IZUMI Yuki HOSHINO Kenji HOSOYA Satoshi TAKAGI Masahiro OKUMURA |
| |
| Institution: | 1)Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060–0818, Japan;2)Veterinary Teaching Hospital, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060–0818, Japan |
| |
| Abstract: | The present study involved the isolation and characterization of canine tumor endothelial
cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following
FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and
histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like
morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and
Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma
exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7
expression was not detected in TECs and tumor cells derived from a hepatocellular
carcinoma. Understanding TEC specific traits may be important in the development of more
efficacious anti-angiogenic therapies that do not induce adverse effects. |
| |
| Keywords: | angiogenesis canine tumor endothelial cell |
|
|
