汾河太原段沉积物中细菌群落的分布特征

分析汾河太原段沉积物细菌群落结构，探讨细菌群落与典型环境因子的相关关系，丰富河流沉积物细菌群落资料，并为汾河太原段生态健康状况评价提供依据。于2020年10月枯水期，在汾河入城口至清徐段布设11个采样断面，采集汾河太原段表层沉积物，共采集到10份样本。采用Illumina高通量测序技术分析沉积物细菌丰度和群落结构，利用主成分分析（PCoA）、冗余分析（RDA）和相关性分析等方法探讨沉积物中细菌群落结构分布特征以及主要影响因素。结果表明，10个断面表层沉积物中5 422个细菌OTUs分属于61门140纲346目580科1 073属。细菌群落结构在断面fh6、fh7、fh8、fh9、fh10、fh11和fh4相似度较高，fh1和fh2断面相似度较高，而fh3断面与其他断面差异显著。除fh3，沉积物细菌优势菌门均为变形杆菌门（Proteobacteria），次优势菌门为绿弯菌门（Chloroflexi），优势菌纲为γ-变形菌纲（Gammaproteobacteria）。Pearson相关性分析表明，TN、OM和Pb与OTUs、Shannon指数和Simpson指数相关性显著。TN、OM和Pb...

Distribution of the Bacterial Community in Sediments of the Taiyuan Section of Fenhe River

In present study, Illumina high-throughput sequencing technology was used to analyze bacterial abundance and community structure in the surface sediments of Taiyuan section of Fenhe Rvier. Principal co-ordinates analysis (PCoA), redundancy analysis (RDA) and correlation analysis were applied to explore the distribution characteristics of bacterial communities and the main influencing factors.The results show that 5422 bacterial OTUs in surface sediment of 10 sections belonged to 61 phyla, 140 classes, 346 ordines, 580 familiae and 1073 genera. The similarity of bacterial community structure was higher in sample section fh6, fh7, fh8, fh9, fh10, fh11 and fh4, and then between fh1 and fh2, but there were significant differences between fh3 and other sections. The dominant phylum of sediment bacteria was Proteobacteria, and the secondary phylum dominant was Chloroflexi. Besides, Gammaproteobacteria was the dominant class among detected bacterial community structures. Pearson correlation analysis showed that TN, OM and Pb were significantly correlated with OTUs, Shannon index and Simpson index, indicating that TN, OM and Pb were the main influence factors of bacterial community structure. RDA and Spearman analysis showed that Proteobacteria was significantly positively correlated with TN and OM (P<0.01) and positively correlated with Pb and Ni (P<0.05); Chloroflexi was significantly negatively correlated with Cr (P<0.01) and significantly positively correlated with OTC and TC (P<0.01); Actinobacteria was negatively correlated with TN, OM and Cu (P<0.05); Firmicutes was negatively correlated with pH and positively correlated with TP (P<0.05). The results of this study can provide data support for understanding the distribution characteristics of the bacterial community in the sediments of Taiyuan section of Fenhe River, and identifying the environmental impact factors.