| 4种体色柞蚕品种遗传关系的RAPD分析 |
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| 引用本文： | 刘彦群,鲁成,向仲怀.4种体色柞蚕品种遗传关系的RAPD分析[J].蚕业科学,2006,32(1):20-24. |
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| 作者姓名： | 刘彦群 鲁成 向仲怀 |
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| 作者单位： | 西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716;沈阳农业大学生物科学与技术学院,沈阳,100161;西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716 |
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| 基金项目： | 国家自然科学基金项目(编号30170719)，教育部重点项目(编号02727) |
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| 摘 要： | 利用随机扩增多态DNA(RAPD)标记技术,对分别属于4种体色系统的13个柞蚕品种进行了基因组DNA多态性分析。利用筛选出的33个随机引物扩增出265条DNA带,其中多态性带227条,占85.66%。根据扩增结果计算品种间的单匹配相似系数,用Ne ighbor-Join ing法构建了聚类树状图。4种体色13个品种并没有按所属体色系统聚在一起,即品种的遗传聚类划分与体色之间的相关性不大,表明柞蚕体色并不能真实反映品种间的遗传关系。
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| 关 键 词： | 柞蚕 品种 体色 遗传关系 随机扩增多态性DNA |
| 文章编号： | 0257-4799(2006)01-0020-05 |
| 收稿时间： | 2005-07-07 |
| 修稿时间： | 2005年7月7日 |
Genetic Relationships of Antheraea pernyi Cultivars From Four Skin Color Based on RAPD Markers |
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| LIU Yan-Qun,LU Cheng,XIANG Zhong-Huai.Genetic Relationships of Antheraea pernyi Cultivars From Four Skin Color Based on RAPD Markers[J].Acta Sericologica Sinica,2006,32(1):20-24. |
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| Authors: | LIU Yan-Qun LU Cheng XIANG Zhong-Huai |
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| Institution: | 1. Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing 400716, China;2. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | The genomic DNA polymorphism of 13 Antheraea pernyi cultivars from 4 larva skin color was detected with RAPD markers.Thirty-three primers selected in this study produced 265 DNA bands,and 227 of which(85.66%) were polymorphic.The simple matching coefficients of similarity were calculated based on RAPD data,and a dendrogram was constructed by Neighbor-Joining mothod.Thirteen cultivars from 4 larva skin color were not clustered by its larva skin color,suggesting that there was no significant correlation between the larva skin color series and the genetic types. |
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| Keywords: | Antheraea pernyi Cultivar Skin color Genetic relationship RAPD |
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