Transgenic ‘Mailing 26’ apple expressing the attacin E gene has increased resistance toErwinia amylovora |
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| Authors: | John L Norelli Herb S Aldwinckle Luis Destéfano-Beltrán Jesse M Jaynes |
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| Institution: | (1) Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, 14456-0462 Geneva, NY, USA;(2) Department of Biochemistry, Louisiana State University, 70803 Baton Rouge, LA, USA;(3) Present address: Centro International de Agricultura Tropical, P.O. Box 6713, Cali, Colombia;(4) Present address: Demeter Biotechnologies, Limited, Research Triangle Park, P.O. Drawer 14388, 27709, NC, USA |
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| Abstract: | Summary Apple (Malus domestica) transgenic T1 was obtained byAgrobacterium tumefaciens-mediated transformation of Malling 26 rootstock using the plasmid binary vector pLDB 15. pLDB 15 contains within its T -DNA
a gene encoding the lytic protein attacin E. The integration of the attacin E gene into the apple genome was confirmed by
Southern analysis. Northern analysis indicated the presence of an attacin E mRNA in plants inoculated withErwinia amylovora. After inoculation ofin vitro grown plants of T1, Malling 26, and Malling 7 (resistant control) withE. amylovora, the loglo of the inoculum concentration lethal to 50% of the plants was 5.4, 4.4, and 5.6, respectively. In greenhouse trials
for resistance to fire blight, T1 was significantly more resistant than ‘Mailing 26’. |
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| Keywords: | apple attacin transformation resistance fire blight Erwinia amylovora Malus domestica |
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