中国荷斯坦牛白细胞粘附缺陷病遗传分析

荷斯坦牛白细胞粘附缺陷病（BLAD）是一种遗传性免疫缺陷病，患病牛出生后，生长发育差，绝大多数在1年内死亡，且不具繁殖和哺育能力。该病的遗传基础为CD18基因编码区383位的A／G点突变导致白细胞表面的β2整合素表达缺陷。目前欧美等奶牛业发达国家都已经建立了完善的BLAD分子检测方法和跟踪监控体系。中国长期从国外进口荷斯坦公牛精液、胚胎或活体，由此可能引进了BLAD基因。本研究采用PCR—RFLP方法对116头可疑中国荷斯坦牛进行了检测．确定了2头BLAD携带者公牛和8头携带者母牛．未发现隐性纯合个体．

Identification of Bovine Leukocyte Adhesion Deficiency in Chinese Holstein

Bovine leukocyte adhesion deficiency(BLAD) is an autosomal recessive disease in Holstein cattle.It is caused by a point mutation from A to G at the site of 383 of the bovine integrin beta-2 subunit(CD18).So far,many countries have established their own methods to identify the carriers of BLAD and monitor the occurrence of the genetic disease in their population.Since China has imported Holstein bulls from other countries,so that BLAD may be introduced and spread in China.In current study,with PCR-RFLP,2 and 8 carriers of BLAD were identified in all of 68 at-risk Chinese Holstein bulls and 48 cows.