Effect of gamma radiation on growth and lignin content in Brachypodium distachyon |
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Authors: | Man Bo Lee Dae Yeon Kim Woong Bae Jeon Min Jeong Hong Yong Jin Lee Odgerel Bold Ji Hee Jang Yun Jeong Kim Si Yong Kang Dong Sub Kim Jin Baek Kim Jae Buhm Chun Yong Weon Seo |
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Institution: | 122. College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of Korea 222. Department of Food and Plant Breeding Research, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea
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Abstract: | Brachypodium distachyon has been highlighted as a model monocot plant with small genome and short life cycle. Biofuels are being developed as renewable energy sources to replace fossil fuels. Bioethanol production is negatively correlated with lignin content. Here, Brachypodium was acutely or chronically irradiated at doses of 50, 100, 150, 200, and 250 Gy. The effect of radiation on plant growth and generation of mutant populations was explored. The lethal effect of radiation was higher in acutely irradiated M0 populations. A dose-dependent negative effect in plant height, tiller number, floral spikelet, and total seed number was observed, with a positive effect in days to heading. The phenotype of 1,773 M1 plants was evaluated, with 417 plants being selected to construct the M2 population. The 31 M2 plants that showed the least staining with phloroglucinol were selected. These mutants could be useful materials for studies such as identification of nucleotide substitutions in genes involved in lignin biosynthesis pathway, monitoring of mutant physiological traits, and evaluation of fitness for bioethanol production. As biological resources, the M2 populations generated in this work will contribute to studies of functional genomics of Brachypodium and to the breeding of grass crops. |
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