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Differentiation between antibodies to serotypes 1 and 2 infectious bursal disease viruses in chicken sera
Authors:N M Ismail  Y M Saif
Affiliation:Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Abstract:The usefulness of the virus neutralization (VN) test, the enzyme-linked immunosorbent assay (ELISA), and the agar gel precipitin (AGP) test in differentiating antibodies to infectious bursal disease virus serotypes 1 and 2 was investigated. Sera examined were from chickens that were challenged with live virus or inoculated with inactivated oil-emulsion IBDV vaccines or were both challenged and inoculated. Antibodies to serotypes 1 and 2 were differentiated by the VN test but not by the ELISA, and the AGP test was less than satisfactory.
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