大白菜子叶离体培养再生植株

 探讨了植物激素、AgNO₃ 和琼脂浓度对大白菜子叶培养芽再生和植株再生的影响。结果表明:优化培养基条件为MS 培养基中加入5 mg / L BA、0. 5 mg/ L NAA、2 mg/ L AgNO₃ 和1. 2%~ 1. 6 %琼脂。培养过程中, 子叶的乙烯释放量对芽再生起很重要的作用, 具有高芽再生率的基因型或高浓度琼脂的培养条件下, 子叶产生的乙烯量较少。培养基中有AgNO₃ 存在时, 尽管子叶的乙烯释放量增加, 芽再生率也有提高。对子叶的乙烯释放量和不定芽再生的关系进行了讨论。

Plant Regeneration from Cotyledonary Explants of Chinese Cabbage Cultured in Vitro

Effect of hormone combinations, AgNO 3 and agar concentration on shoot and plant regeneration from cotyledonary explants of Chinese cabbage was described. Maximum shoot regeneration was obtained in MS medium containing 5 mg/L BA, 0.5 mg/L NAA, 2 mg/L AgNO 3 and solidified with 1.2%-1.6% agar. Ethylene production from cultured explants seemed to play important role in shoot regeneration. Explants of cultivars with high regeneration frequency or if cultured on the medium solidified with higher concentration of agar generally showed low levels of ethylene production. However, AgNO 3, which also enhanced shoot induction, resulted in an increase in ethylene production. The possible interaction between ethylene and shoot regeneration is discussed.