| 哺乳动物肠上皮细胞的原代培养 |
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| 引用本文： | 刘芳宁,张彦明. 哺乳动物肠上皮细胞的原代培养[J]. 动物医学进展, 2007, 28(4): 53-57 |
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| 作者姓名： | 刘芳宁 张彦明 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西杨陵,712100;西北农林科技大学动物科技学院,陕西杨陵,712100 |
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| 摘 要： | 肠上皮细胞是由多功能干细胞分化成的一个高度组织化系统.从离体的肠组织中分离的肠隐窝单位或肠上皮细胞可在数小时内保持高度活力,但要长期(达到10 d)原代培养肠上皮细胞仍然很困难.文章对肠上皮细胞的分离、鉴定、原代培养所需维持培养基和生长培养基的设计及培养条件等方面进行了综述.
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| 关 键 词： | 原代培养 哺乳动物 小肠 结肠 上皮组织 |
| 文章编号： | 1007-5038（2007）04-0053-05 |
| 收稿时间： | 2006-12-19 |
| 修稿时间： | 2006-12-19 |
Primary Culture of Mammalian Intestinal Epithelial Cells |
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| LIU Fang-ning,ZHANG Yan-ming. Primary Culture of Mammalian Intestinal Epithelial Cells[J]. Progress In Veterinary Medicine, 2007, 28(4): 53-57 |
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| Authors: | LIU Fang-ning ZHANG Yan-ming |
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| Abstract: | Epithelial cells lining the digestive tract represent a highly organized system built up by multipotent stem cells.Long-term primary culture of intestinal cells(up to 10 d) is still difficult despite progress in isolation methodologies and manipulation of the cell microenvironment.In this article,the isolation of viable of epithelial cells,the identification,construction of maintenance and growth media,cell culture conditions were discussed.The main aim is to show the difficulty of primary culture of mammalian intestinal epithelial cells,at the same time,to take some signs or experiences for intestinal epithelial cell culture. |
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| Keywords: | primary culture mammals small intestine colon epithelium |
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