培养条件对奶牛金葡菌5型荚膜多糖产量的影响

奶牛乳腺炎是奶牛生产上的重要疾病，不仅影响奶牛的产奶量、降低牛奶品质，而且严重威胁消费者健康，已成为制约奶牛业发展的主要原因之一。据国内外相关资料显示，50％以上的奶牛乳腺炎是由金葡菌（Staphylococcus aureus,S.aureus）所引起的。研究发现，94％～100％的从患乳腺炎奶牛乳汁中分离到的金葡菌菌株表面带有多糖物质构成的保护性荚膜荚膜具有11种血清型，

The Effect of Culture Condition on Type 5 Capsular Polysaccharide Production of Staphylococcus aureus from Diary Cattle

Objective]The effect of different culture conditions on type 5 capsular polysaccharide production of Staphylococcus aureus from diary cattle was studied to provide simple way for CP production and preparation and laid foundation for carrying out new polysaccharide vaccine research.Method]Staphylococcus aureus was isolated from milk sample of sick dairy cattle and capsular polysaecharide serotypes were identified.Type 5 capsular polysaccharide was cultured on BHI,solid columbia and med110 culture media.Glucose and lactose were taken as carbon sources for every culture media in solid and liquid state.Therefore 9 different culture conditions were taken to study the effect of cuhure conditions on capsular polysaccharide production.Result]Different culture conditions indicated that compared with columbia culture media,BHI culture media could decline capsular polysaccharide production and mod110 culture media could increase capsular polysaccharide production.While for same culture media,solid culture media was better for capsular polysaccharide production,meanwhile,taken lactose as carbon source could increase capsular polysaccharide production.