| 营养环境对微小亚历山大藻C4生长和产毒的影响 |
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| 引用本文： | 王亚军,余新威,方力,刘其林,任斌,罗红宇.营养环境对微小亚历山大藻C4生长和产毒的影响[J].水产学报,2017,41(10):1588-1598. |
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| 作者姓名： | 王亚军 余新威 方力 刘其林 任斌 罗红宇 |
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| 作者单位： | 1. 浙江海洋大学食品与医药学院,浙江省海产品健康危害因素关键技术研究重点实验室,浙江舟山 316022;2. 浙江省舟山市疾病预防控制中心,浙江舟山,316021 |
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| 基金项目： | 舟山市科技局一般类海洋项目（2013C31070）；国家星火计划项目（2015GA700094）；浙江省大学生科技创新活动计划暨新苗人才计划项目（2015R411059） |
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| 摘 要： | 为考察营养成分以及培养基础液再利用对一株微小亚历山大藻的生长和产毒的影响,采用单因素实验分别研究了氮(NaNO_3)、磷(NaH_2PO_4)、微量元素(FeCl_3、Na2EDTA、CuSO_4、Na_2MoO_4、ZnSO_4、CoCl_2、MnCl_2)、维生素(Vitamin B12、Vitamin H、Vitamin B1)、碳(NaHCO_3)的不同含量以及海水(培养基础液)利用方式(不循环与循环利用)对微小亚历山大藻C4生长、毒素含量(μmol/L)与毒素组成的影响。结果显示,氮、磷浓度对C4藻的毒素总含量(μmol/L)有显著影响,其他因素影响均不显著;氮、磷、碳及微量元素的浓度对GTX1/4(GTX1+GTX4)在总毒素(GTX1+GTX2+GTX3+GTX4)中所占比例均影响显著,而维生素浓度和海水循环利用对GTX1/4所占比例影响均不明显。氮浓度在0~883μmol/L范围内,毒素含量与氮浓度呈正相关关系,氮浓度进一步增加,毒素含量没有明显变化;磷浓度在0~145.2μmol/L范围内增加,毒素含量先增后降,最终保持稳定的趋势。最佳的产毒条件为氮883μmol/L,磷18.15μmol/L,微量元素为f/2海水培养基中微量元素的0.5倍,碳不添加。
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| 关 键 词： | 微小亚历山大藻C4 膝沟藻毒素 营养因子 麻痹性贝类毒素 |
| 收稿时间： | 2016/9/26 0:00:00 |
| 修稿时间： | 2017/3/10 0:00:00 |
Influences of nutrient environment on growth and toxin productivity of Alexandrium minutum C4 |
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| WANG Yajun,YU Xinwei,FANG Li,LIU Qilin,REN Bin and LUO Hongyu.Influences of nutrient environment on growth and toxin productivity of Alexandrium minutum C4[J].Journal of Fisheries of China,2017,41(10):1588-1598. |
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| Authors: | WANG Yajun YU Xinwei FANG Li LIU Qilin REN Bin and LUO Hongyu |
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| Institution: | Key Laboratory of the Key Techniques on Health Hazard Factors of Seafood in Zhejiang Province, Institute of Food and Pharmacy, Zhejiang Ocean university, Zhoushan 316022, China,Zhoushan Center for Disease Control and Prevention, Zhoushan 316021, China,Zhoushan Center for Disease Control and Prevention, Zhoushan 316021, China,Key Laboratory of the Key Techniques on Health Hazard Factors of Seafood in Zhejiang Province, Institute of Food and Pharmacy, Zhejiang Ocean university, Zhoushan 316022, China,Key Laboratory of the Key Techniques on Health Hazard Factors of Seafood in Zhejiang Province, Institute of Food and Pharmacy, Zhejiang Ocean university, Zhoushan 316022, China and Key Laboratory of the Key Techniques on Health Hazard Factors of Seafood in Zhejiang Province, Institute of Food and Pharmacy, Zhejiang Ocean university, Zhoushan 316022, China |
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| Abstract: | In order to grasp the influences of nutrients and sea water reuse on growth and toxin productivty of the Alexandrium minutum, single factor experiments were used to observe the influences of the concentrition of nitrogen (NaNO3), phosphorus (NaH2PO4), trace element (FeCl3, Na2EDTA, CuSO4, Na2MoO4, ZnSO4, CoCl2, MnCl2), vitamin (Vitamin B12, Vitamin H, Vitamin B1), carbon (NaHCO3) and the style of sea water (new or reuse) on the growth, toxin content (μmol/L) and toxin constitution ofAlexandrium minutum C4. The results showed that nitrogen and phosphorus had a significant effect on the total toxin content (μmol/L), while others had non-significant effect (μmol/L); nitrogen, phosphorus, trace element and carbon had a significant effect on the ratio of GTX1/4(GTX1+GTX4) to total toxin content(GTX1+GTX2+GTX3+GTX4), while vitamin and sea water reuse had non-significant effect on that ratio. There was a positive correlation between the concentration of toxin and nitrogen when the nitrogen concentrition was between 0 and 883 μmol/L, and then toxin content stayed invariant with the increase of nitrogen. However, with the increase of phosphorus concentrition from 0 to 145.2 μmol/L, the toxin content increased at first, then reduced,and stayed invariant in the end.The optimal conditions for the toxin production were nitrogen 883 μmol/L, phosphorus 18.15 μmol/L, trace element one half of that in the f/2 medium and carbon free. |
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| Keywords: | Alexandrium minutum C4|gonyautoxin|nutrient|paralytic shellfish toxin |
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