| 柠条锦鸡儿DNA提取及AFLP反应体系的建立 |
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| 引用本文： | 王赞,高洪文,韩建国.柠条锦鸡儿DNA提取及AFLP反应体系的建立[J].草地学报,2005,13(2):126-129. |
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| 作者姓名： | 王赞 高洪文 韩建国 |
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| 作者单位： | 1. 中国农业大学草地所, 北京, 100094;2. 中国农业科学院畜牧所, 北京, 100094 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以柠条锦鸡儿(Caragana korshinskii)种子为材料,利用改进的酚一氯仿法,提取到高质量的DNA,建立柠条锦鸡儿AFLP银染反应体系。结果表明:首次在柠条锦鸡儿DNA提取、酶切连接、预扩增和选择性扩增等实验过程取得成功;得到清晰的柠条锦鸡儿AFLP指纹图谱;为建立柠条锦鸡儿基因库、品种选育以及亲缘关系的研究提供参考。
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| 关 键 词： | 柠条锦鸡儿 DNA提取 AFLP |
| 文章编号： | 1007-0435(2005)02-0126-04 |
| 收稿时间： | 2005-03-29 |
| 修稿时间： | 2005年3月29日 |
DNA Extraction Method and Profile of AFLP Fingerprint of Caragana korshinskii |
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| WANG Zan,GAO Hong-wen,HAN Jian-guo.DNA Extraction Method and Profile of AFLP Fingerprint of Caragana korshinskii[J].Acta Agrestia Sinica,2005,13(2):126-129. |
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| Authors: | WANG Zan GAO Hong-wen HAN Jian-guo |
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| Institution: | 1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China;2. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China |
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| Abstract: | The extraction of DNA with phenol-cholorofore method from Caragana korshinskii seed and AFLP fingerprint profile of C. korshinskii were studied. The high resolution AFLP fingerprints were clear,showing the success in the DNA extraction, restriction-ligase reaction,pre-amplification and silver staining techniques.The results could supply references to the gene pool establishment, selection and breeding of cultivars and genetic relationships in C. korshinskii. |
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| Keywords: | Caragana korshinskii DNA extraction AFLP |
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