| 早食李幼胚离体培养的研究 |
| |
| 引用本文： | 余小玲,何业华,韩景忠,谢志亮,彭兵. 早食李幼胚离体培养的研究[J]. 安徽农业科学, 2010, 38(19): 9981-9982,9987. DOI: 10.3969/j.issn.0517-6611.2010.19.020 |
| |
| 作者姓名： | 余小玲 何业华 韩景忠 谢志亮 彭兵 |
| |
| 作者单位： | 华南农业大学园艺学院,广东广州,510642;广东外语外贸大学南国商学院,广东广州,510420;华南农业大学园艺学院,广东广州,510642 |
| |
| 摘 要： | [目的]探讨早食李幼胚离体培养的影响因素。[方法]以不同发育时期的早食李胚为试材,研究了不同胚龄、不同培养基、GA3浓度对离体胚培养的影响。[结果]花后26～41d的幼胚细小、胚乳尚存,经连续3代培养仍未发生任何变化;从花后48d开始胚乳已经消失,幼胚不断发育并逐渐充满整个种腔,接种在MS基本培养基上后其成活率高达77%以上,培养初代即可启动;4.5%的花后65d幼胚已能培养成苗,此后成苗率随着胚龄增加而升高,至果实成熟时成苗率可达26.00%,并在培养初代可产生愈伤组织;花后65～83d的幼胚则需经2～3代以上才能诱导出愈伤组织。胚（花后88d）在WPM培养基的成苗率比MS培养基上高1倍左右;在同一基本培养基上,添加BA、KT或2,4-D后,成苗过程受到抑制。用1000mg/LGA3处理种子24h后能显著提高胚的成苗率。[结论]为建立早食李胚胎抢救技术及杂交育种奠定实验基础。
|
| 关 键 词： | 早食李 幼胚 离体培养 胚龄 |
Study on Culturing of Prunus salicina cv.Zaoshi Embryoes |
| |
| YU Xiao-ling et al. Study on Culturing of Prunus salicina cv.Zaoshi Embryoes[J]. Journal of Anhui Agricultural Sciences, 2010, 38(19): 9981-9982,9987. DOI: 10.3969/j.issn.0517-6611.2010.19.020 |
| |
| Authors: | YU Xiao-ling et al |
| |
| Affiliation: | YU Xiao-ling et al(College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642) |
| |
| Abstract: | [Objective] The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryoes in vitro.[Method] Different age,culturing base and GA3 which affected the culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the ... |
| |
| Keywords: | Prunus salicina cv.Zaoshi Embryoes Culture Age of embryo |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
