玉米灰斑病菌人工接种方法

玉米灰斑病是严重威胁玉米生产的叶部病害。采用喷雾、菌液灌心、高粱粒灌心、注射4种方法进行接种,以探索抗病资源鉴定中更为简便、易行、可靠的田间人工接种方法。对玉米叶部病害喷雾是常用的接种方法,对玉米灰斑病菌高粱粒灌心方法不但有利于保湿,而且便于操作,是抗性鉴定时的首选方法。在植株11～12叶期(喇叭口期)应用高粱粒灌心的接种方法,可获得理想的寄主发病效果。

Inoculation Method of Gray Leaf Spot Pathogen

The gray leaf spot(GLS) is a significant leaf disease in maize.Four inoculation method including spraying,inoculate inocula into leaf whorl(inoculate inocula),inoculate sorghum seed with inocula into leaf whorl(inoculate sorghum seed) and injection have been used in this article.This research studied handy,easy and authentic artificial inoculation method of resistant identification in field.The spraying method is common inoculation technique of maize leaf disease.But the method of that,inoculate sorghum seed with inocula into leaf whorl is the first resistant identification of GLS pathogen because it is better in the heat humidity and operation.Sorghum seed with inocula was inoculated into leaf whorl of plants at 11-12 leaf stage of plant.The increased disease incidence was observed in the fields.