| 猪圆环病毒复合PCR检测方法的建立及应用 |
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| 引用本文： | 王宏魁,彭志锋,孙彦婷,徐雪,刘春生,刘慧敏,王川庆. 猪圆环病毒复合PCR检测方法的建立及应用[J]. 河南农业科学, 2009, 0(1) |
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| 作者姓名： | 王宏魁 彭志锋 孙彦婷 徐雪 刘春生 刘慧敏 王川庆 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 摘 要： | 根据GenBank中PCV2和PCV1的基因组序列设计3条引物,并建立了检测PCV2的复合PCR方法。用该方法对河南省7个地市45个猪场的156份样品进行了检测,检出阳性病料98份,阳性率63%;阳性猪场38个,阳性率84%,表明该方法特异性及敏感性高,可用于临床诊断。
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| 关 键 词： | 猪圆环病毒2型 复合PCR 检测 |
Development and Application of Multi-PCR for the Detection of Porcine Circovirus Type 2(PCV2) |
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| WANG Hong-kui,PENG Zhi-feng,SUN Yan-ting,XU Xue,LIU Chun-sheng,LIU Hui-min,WANG Chuan-qing. Development and Application of Multi-PCR for the Detection of Porcine Circovirus Type 2(PCV2)[J]. Journal of Henan Agricultural Sciences, 2009, 0(1) |
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| Authors: | WANG Hong-kui PENG Zhi-feng SUN Yan-ting XU Xue LIU Chun-sheng LIU Hui-min WANG Chuan-qing |
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| Affiliation: | College of Animal Science and Veterinary Medicine;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | According to the published sequences of porcine circovirus type 2 (PCV2) and type 1 ( PCV1),three primers were designed and a multiplex PCR method was established for detecting PCV2.A total of 156 samples collected from 45 pig farms in Henan province were detected.The results showed that 98 samples were positive and the positive rate of PCV2 was 63%.38 farms were positive and the positive rate was 84%.The data indicated that this method was rapid,specific and sensitive for the detection of PCV2,which can be... |
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| Keywords: | Porcine circovirus type 2 (PCV2) Muti-PCR Detection |
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