| 猪卵母细胞体外成熟研究 |
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| 引用本文： | 郝子悦,刘忠慧. 猪卵母细胞体外成熟研究[J]. 安徽农业科学, 2010, 38(17): 9011-9012,9082 |
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| 作者姓名： | 郝子悦 刘忠慧 |
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| 作者单位： | 江苏畜牧兽医职业技术学院,江苏泰州,225300;江苏畜牧兽医职业技术学院,江苏泰州,225300 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2006)第5-3号]资助 |
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| 摘 要： | [目的]探讨猪卵母细胞体外成熟的最佳培养条件。[方法]以NCSU-23为基础培养基,研究不同激素组合和培养时间（36、40、44和48 h）对猪卵母细胞体外成熟的影响。[结果]猪卵母细胞体外培养44 h后,成熟率最高,为81.4%;猪卵母细胞在成熟培养液培养22h后,再在无激素培养液中继续培养22 h时,成熟率最高;添加性腺激素的组成熟率显著高于不添加组,差异显著（P〈0.05）。[结论]在体外成熟培养过程中,添加性腺激素能显著提高猪卵母细胞成熟率。
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| 关 键 词： | 猪 卵母细胞 体外成熟 |
Maturation of Porcine Oocytes in vitro |
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| HAO Zi-yue et al. Maturation of Porcine Oocytes in vitro[J]. Journal of Anhui Agricultural Sciences, 2010, 38(17): 9011-9012,9082 |
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| Authors: | HAO Zi-yue et al |
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| Affiliation: | HAO Zi-yue et al(Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300) |
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| Abstract: | [Objective]To explore the optimum conditions of the porcine oocytes maturation in vitro.[Method]NCSU-23 medium was used as basic medium.The effects of different maturation times(36,40,44 and 48 h) and different hormone combinations on porcine oocyte maturation in vitro were studied.[Result]After cultivation for 44 h in vitro,the nuclear maturation rate was up to 81.4%.The nuclear maturation rate was the highest when cultivating for 22 h in mature medium and then cultivating for 22 h in no gonadotropin mediu... |
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| Keywords: | Pig Oocyte Maturation in vitro |
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