响应面法优化番茄早疫病拮抗菌WL07发酵条件

应用响应面法对番茄早疫病拮抗菌WL07的培养基组成及培养条件进行优化。通过Plackett-Burman设计试验,从蔗糖、碳酸钙、硫酸铵、硫酸亚铁、酵母膏、装液量、初始pH等影响因素中筛选影响拮抗菌WL07发酵的显著因素。Plackett-Bur-man设计试验结果表明影响拮抗菌WL07发酵的显著因素分别为初始pH和蔗...

Fermentation Condition Optimization for Altermaria solani Antagonistic Microorganisms WL07 using Response Surface Methodology

Response surface method was applied to optimize the composition of culture medium and culture conditions of Altermaria solani antagonistic microorganisms WL07.Plackett-Burman experiment was applied to screen the significant factors which could affect on WL07 biological fermentation,from sucrose,calcium carbonate,ammonium sulfate,etc.Plackett-Burman experimental results showed that two significant factors were sucrose and initial pH.The steepest ascent method had determined the concentration of 3% sucrose,the initial pH6.5 as the center combination design experiment center.The optimal fermentation conditions of WL07 could be determined by using the response surface method,when sugar concentration 30.1 g·L-1,initial pH6.2,antibacterial circle diameter was about 1.27-fold increase compared with that using the original fermentation conditions,by 22.01 mm increased to 28.00 mm,increased nearly 6 mm.