接触二甲苯实验人员淋巴细胞染色体畸变分析

目的:研究二甲苯对实验人员外周血淋巴细胞染色体畸变的影响.方法:外周血培养制备染色体标本,分析外周血淋巴细胞染色体畸变.结果:二甲苯接触组的染色体数目畸变细胞率(11.3%)高于对照组(6.8%)(P＜0.05);结构畸变细胞率(包括裂隙)(3.57%)高于对照组(2.6%)(P＜0.05)并且呈剂量效应关系;接触组的未成熟着丝粒分离细胞率(1.85%)与对照组(1.34%)相比无显著差异(P＞0.05).结论:二甲苯接触导致外周血淋巴细胞染色体畸变增加;二甲苯接触是否诱导未成熟着丝粒分离有待进一步深入研究.

Chromosome Aberration Analysis of Peripheral Lymphocytes in Workers Exposed to Xylene

Objective:To study the effects of xylene on chromosome aberration of peripheral lymphocytes in workers.Methods: Make chromosomal samples with peripheral lymphocyte culture and analyze chromosome aberrations of lymphocytes.Results:The numerically aberrated cell rate of the workers exposed by xylene(11.3%)was significantly higher than of the controls(6.8%)(P<0.05);and the structural aberrated cell rate(included gaps)(3.57%)was significantly higher than that of the controls(2.6%)(P<0.05)in a dose-effect ralationship manner;the premature centromere division cell rate of the xylene exposed workers(1.85%)was not significantly higher than that of the contols(1.34%)(P>0.05).Conclusion:Xylene exposure resulted in an increase of chromosome aberrations of the peripheral lymphocytes;whether xylene exposure could induce premature centromere division or not deserves further studies.