| 鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析 |
| |
| 引用本文： | 易春华,潘杰,付薇,颜健华,徐贤坤,熊毅. 鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析[J]. 安徽农业科学, 2009, 37(25) |
| |
| 作者姓名： | 易春华 潘杰 付薇 颜健华 徐贤坤 熊毅 |
| |
| 作者单位： | 广西大学动物科学技术学院,广西南宁,530005;广西壮族自治区动物疫病预防控制中心,广西南宁,530001 |
| |
| 摘 要： | [目的]克隆鹅副粘病毒GX1株HN基因与F基因,并进行序列分析。[方法]根据Genbank已发表的鹅副粘病毒GPV-SF02株基因组核苷酸序列设计2对引物,对从广西发病鹅分离到的鹅副粘病毒毒GX1株的HN和F基因进行PCR扩增,将扩增产物与pMD18-T载体连接并测序。[结果]该株副粘病毒株的HN基因和F基因核苷酸序列全长分别为1 716和1 662 bp,与GPV-SF02株的同源性均在97.3%左右,与LaSota株、F48E9株、JS株的同源性为80.3%~97.5%,与M iyadera株的同源性仅84.8%。[结论]分离株GX1株与禽Ⅰ型副粘病毒(A PMV-1)强毒株特征相符,属于A PMV-1基因Ⅶ型。
|
| 关 键 词： | 鹅副粘病毒 HN蛋白基因 F蛋白基因 克隆 序列分析 |
Cloning and Sequence Analysis of HN and F Proteins Encoding Genes from a Strain of Goose Paramyxovirus |
| |
| YI Chun-hua et al. Cloning and Sequence Analysis of HN and F Proteins Encoding Genes from a Strain of Goose Paramyxovirus[J]. Journal of Anhui Agricultural Sciences, 2009, 37(25) |
| |
| Authors: | YI Chun-hua et al |
| |
| Affiliation: | YI Chun-hua et al(College of Animal Science , Technology,Guangxi University,Nanning,Guangxi 530005) |
| |
| Abstract: | [Objective] The study was to clone HN and F genes from GX1 strain of goose paramyxovirus and analyze their sequences.[Method] According to the full nucleotide sequence of GPV-SF02 strain of goose paramyxovirus,two pairs of primers were designed to amplify the HN and F genes from GX1 strain of goose paramyxovirus isolated in diseased goose in Guangxi Zhuang Autonomous Region;the amplified products were ligated into pMD18-T vector and sequenced.[Result] HN and F genes of this strain tested were 1 716 and 1 66... |
| |
| Keywords: | Goose paramyxovirus HN protein gene F protein gene Cloning Sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
