| 大豆幼嫩子叶离体培养及辐照处理配合基因枪遗传转化研究 |
| |
| 引用本文： | 刘智宏,胡张华,郎春秀,黄锐之,陈锦清. 大豆幼嫩子叶离体培养及辐照处理配合基因枪遗传转化研究[J]. 核农学报, 2001, 15(5): 282-285 |
| |
| 作者姓名： | 刘智宏 胡张华 郎春秀 黄锐之 陈锦清 |
| |
| 作者单位： | 浙江省农业科学院原子能利用研究所 |
| |
| 基金项目： | 浙江省九五重点科技攻关资助项目 |
| |
| 摘 要： | 3年来共收集 40份栽培大豆和野生大豆材料 ,进行幼嫩子叶的离体培养 ,其中 3个品种获得较高的再生植株频率。辐照处理配合基因枪介导转基因研究表明 ,5Gyγ射线辐照处理能明显提高抗性愈伤的频率。GUS检测抗性愈伤组织 ,证明外源基因已导入大豆细胞 ,并在其中表达
|
| 关 键 词： | 大豆 基因枪 辐照 转化 |
| 文章编号： | 10000-855(2001)05-0282-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 1999-02-09 |
IN VITRO CULTURE OF SOYBEAN IMMATURE COTYLEDONS AND GENETIC TRANSFORMATION VIA BIOLISTIC SYSTEM |
| |
| Abstract: | soybean ( Glycine max (L.)Merr.) cultivars were collected to study in vitro culture of immature cotyledons.Regenerated plants of 3 cultivars were obtained and one cultivar was used to study the effect of irradiation treatment on stable genetic transformation rate.0,5,10,20 Gy were applied before or after bombardment with Bio Rad system.It was found that 5 Gy was the effective dose to raise the ratio of cotyledons with Km R calli.GUS enzyme activity showed that foreign genes had been integrated into genome of soybean callus. |
| |
| Keywords: | soybean transformation biolistic system irradiation treatment |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
|
点击此处可从《核农学报》下载全文 |
|
