| 亚麻与红麻的原生质体融合 |
| |
| 引用本文： | 王丽爽,易继财,张宗申. 亚麻与红麻的原生质体融合[J]. 广东农业科学, 2010, 37(4): 191-193 |
| |
| 作者姓名： | 王丽爽 易继财 张宗申 |
| |
| 作者单位： | 1. 大连工业大学生物与食品工程学院,辽宁大连,116034
2. 华南农业大学生命科学学院,广东广州,510642 |
| |
| 基金项目： | 辽宁省教育厅科学技术研究重点实验室项目 |
| |
| 摘 要： | 将亚麻与红麻的愈伤组织在水解酶的作用下分离出原生质体,然后利用PEG法使这2种原生质体融合。结果表明,用2%纤维素酶+1%果胶酶+0.55mol/L甘露醇处理材料,酶解6h后,可以获得大量游离的、圆球形的原生质体;利用40%PEG6000及0.3mol/LCaCl2溶液(含9%甘露醇,pH9.5)将这2种原生质体融合,可以获得稳定的杂合细胞。
|
| 关 键 词： | 亚麻 红麻 原生质体融合 |
Protoplast fusion of Linum usitatissimum L. and Hibiscus cannabius L. |
| |
| WANG Li-shuang,YI Ji-cai,ZHANG Zong-shen. Protoplast fusion of Linum usitatissimum L. and Hibiscus cannabius L.[J]. Guangdong Agricultural Sciences, 2010, 37(4): 191-193 |
| |
| Authors: | WANG Li-shuang YI Ji-cai ZHANG Zong-shen |
| |
| Abstract: | With the method of enzymolysis.the protoplasts were released from the callus of Linum usitatissimum L and Hibiscus cannabius L.,respectively,and then they were fused by PEG as fusogen.The results showed that spherical protoplasts were available in sufficieut quantities by using 2% cellulase+1%peetinase+0.5 mol/L mannitol to treat the callUS for 6 h. Furthermore,the two different protoplasts could be fused into heterozygous pmtoplasts using 40%PEG fusion liquid and 0.3 mol/L CaCl2 solution(containing 9%mannitol,pH value 9.5),and these heterozygous protoplasts were stable. |
| |
| Keywords: | Linum usitatissimum L. Hibiscus cannabius L. pmtoplast fusion |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
|
点击此处可从《广东农业科学》下载全文 |
|
