| 双单倍体马铃薯染色体加倍的方法 |
| |
| 引用本文： | 朱明凯,程天庆,高湘玲,宋伯符,吴绍岩,田翠萍.双单倍体马铃薯染色体加倍的方法[J].中国马铃薯,1987(2). |
| |
| 作者姓名： | 朱明凯 程天庆 高湘玲 宋伯符 吴绍岩 田翠萍 |
| |
| 作者单位： | 中国农科院蔬莱研究所，中国农科院蔬莱研究所，中国农科院蔬莱研究所，中国农科院蔬莱研究所，中国农科院蔬莱研究所，中国农科院蔬莱研究所 |
| |
| 摘 要： | 本试验研究了马铃薯双单倍体(双单倍体是由四倍体栽培种花药培养产生的)植株加倍的两种方法。第一种方法:在MS培养基内附加NAA 0.1mg/1,而后加入秋水仙素0.2mg/l,用马铃薯双单倍体植株切断接种。经过40天培养,获得了加倍的四倍体植株,加倍频率为63.6%。第二种方法:将马铃薯双单倍体植株的茎,接种在含有2.4-D 1mg/l,萘乙酸0.025mg/l,KT 2mg/l的MS培养基上,在产生愈伤组织后诱导出再生四倍体植株,加倍频率为22.3%。
|
THE METHOD OF DOUBLING CHROMOSOME IN DIHAALOID OF SOLANUM TUBEROSUM |
| |
| Abstract: | Two methods of doubling chromosome in dihaploid(2n=24)developed from anther culture of S.tuberosum(2n=48)were sdudied.First,NAA (naphthalene acetic acid)0.1mg/1 and colchicine 0.2mg/1 were added to MS medium,on which plant segments of dihaploid were inoculated.After 40 days,plants with chromosomes doubled(2n=48)were gained and doubling frequency was 63.6%.Second,stem segments of dihaploid were inoculated on MS medium in which 2.4-D 1mg/1,NAA 0.025mg/1 and KT(Kinetin) 2.0mg/1 were added.Doubled plants were derived from the callus preformed, and doubling frequency was 22.3%. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
|
