Graphical Genotype of Maize Inbred B86 Revealed by RFLPs |
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| Authors: | S. Fahr M. M. Messmer A. E. Melchinger M. Lee W. L. Woodman |
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| Affiliation: | Institut für Pflanzenzüchtung, Saatgutforschung und Populationsgenetik, Universität Hohenheim, P.O. Box 700562, W-7000 Stuttgart 70, Germany;Department of Agronomy, Iowa State University, Ames/IA, 50011, U.S.A. |
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| Abstract: | The objective of this study was to investigate the origin of chromosome segments of maize (Zea mays L.) inbred B86 from its parental inbreds B52 and Oh43 using RFLPS. B86 was selected to combine resistance to the first and second brood of the European corn borer with the favorable agronomic performance of Oh43. RFLP analyses of the three inbreds were performed with three restriction enzymes (EcoRI, EcoRV, HindIII) and 178 DNA probes covering all ten maize chromosomes. For two DNA probes in combination with one enzyme, B86 showed a new RFLP pattern not present in the two parents. Based on results from 119 polymorphic DNA probes, the proportion of the B86 genome contributed by B52 (51.3 %) and Oh43 (48.7 %) was almost equal. |
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| Keywords: | Zea mays RFLPS graphical genotype Ostrinia nubilalis |
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