| 转基因抗除草剂油菜相关基因的PCR检测及其在育种中的应用 |
| |
| 引用本文： | 浦惠明,戚存扣,张洁夫,陈松,陈峰,高建芹,陈新军.转基因抗除草剂油菜相关基因的PCR检测及其在育种中的应用[J].江苏农业学报,2006,22(4):356-361. |
| |
| 作者姓名： | 浦惠明 戚存扣 张洁夫 陈松 陈峰 高建芹 陈新军 |
| |
| 作者单位： | 江苏省农业科学院经济作物研究所,江苏,南京,210014 |
| |
| 基金项目： | 引进国际先进农业科技计划(948计划);江苏省高技术研究发展计划项目 |
| |
| 摘 要： | 以抗草甘膦杂交油菜三系和抗草丁膦杂交油菜二系亲本及其F1杂种为材料,探索建立和优化抗草甘膦的CP4、gox基因和抗草丁膦的bar基因及其Barnase、Barstar基因的PCR检测方法,为转基因抗除草剂油菜的品种选育、F1种子纯度鉴定和转基因油菜的生态安全性研究提供可靠、实用的检测方法。
|
| 关 键 词： | 转基因油菜 抗除草剂基因 PCR检测方法 |
| 文章编号： | 1000-4440（2006）04-0356-06 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006年2月28日 |
PCR Detection of Herbicide-resistance-related Genes in Transgenic Rapeseed and Its Application in Breeding |
| |
| PU Hui-ming,QI Cun-kou,ZHANG Jie-fu,CHEN Song,CHEN Feng,GAO Jian-qin,CHEN Xin-jun.PCR Detection of Herbicide-resistance-related Genes in Transgenic Rapeseed and Its Application in Breeding[J].Jiangsu Journal of Agricultural Sciences,2006,22(4):356-361. |
| |
| Authors: | PU Hui-ming QI Cun-kou ZHANG Jie-fu CHEN Song CHEN Feng GAO Jian-qin CHEN Xin-jun |
| |
| Institution: | Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
| |
| Abstract: | In order to develop a reliable and practical transgene detection method for the breeding of transgenic herbicide-resistant rapeseed,the identification of F_1 seed purity and the researches on ecological safety,glyphosate-resistant three-line hybrid rapeseed and glufosinate-resistant two-line hybrid rapeseed and their F_1 hybrid were used to establish and optimize the PCR method for detection of CP_4,gox genes concerning Glyphosate-resistance,and bar,Barnase and Barstar genes associated with glufosinate-resistance.The status of transgenic crop planted in the world and progress in herbicide-resistant rapeseed research were also reviewed and summarized in the paper. |
| |
| Keywords: | transgenic rapeseed herbicide resistance gene PCR detection method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
