| 牛瑟氏泰勒虫18S rRNA基因的克隆及分子分类学分析 |
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| 引用本文： | 沈岩,许应天,李静,栾杨,杨兴. 牛瑟氏泰勒虫18S rRNA基因的克隆及分子分类学分析[J]. 安徽农业科学, 2009, 37(31): 15175-15176 |
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| 作者姓名： | 沈岩 许应天 李静 栾杨 杨兴 |
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| 作者单位： | 延边大学农学院,吉林龙井,133400;延边大学农学院,吉林龙井,133400;延边大学农学院,吉林龙井,133400;延边大学农学院,吉林龙井,133400;延边大学农学院,吉林龙井,133400 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 为分析牛瑟氏泰勒虫延边株18SrRNA基因序列，根据GenBank上已发表的牛瑟氏泰勒虫18SrRNA基因序列设计2对特异性引物，通过PCR扩增目的基因片段，将扩增产物连接到pMD18一T载体中，经酶切鉴定和PCR鉴定为阳性的重组质粒进行测序，对测序结果用DNAMAN软件时其与GenBmlk上8个虫株相关序列进行同源性比较，建立系统发育树。结果显示，牛瑟氏泰勒虫中国延边株的18SrRNA基因大小为1744bp，瑟氏泰勒虫延边株、兰州株、日本株以及水牛泰勒虫中国株彼此之间亲缘关系最近；瑟氏泰勒虫延边株与突变泰勒虫亲缘关系较远：
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| 关 键 词： | 牛瑟氏泰勒虫 18SrRNA基因 克隆 分子分类学 |
Cloning and Molecular Taxonomy Analysis of 18S rRNA Gene of Cattle Theileria sergenti |
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| SHEN Yan et al. Cloning and Molecular Taxonomy Analysis of 18S rRNA Gene of Cattle Theileria sergenti[J]. Journal of Anhui Agricultural Sciences, 2009, 37(31): 15175-15176 |
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| Authors: | SHEN Yan et al |
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| Affiliation: | SHEN Yan et al (Agricultural College of Yanbian University,Longjing,Jilin 133400) |
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| Abstract: | To analyze 18S rRNA nucleotide sequence of cattle Theileria sergenti,two pairs of specific primers were deigned according to 18S rRNA gene of cattle Theileria sergenti sequences published on GenBank.18S rRNA gene was amplified by PCR and cloned into pMD18-T vector.Positive clones were identified by PCR screening and restriction digestion enzyme.The phylogenetic tree was inferred based on 18S rRNA sequence of Yanbian and the other eight species of Theileria available on GenBank.Sequencing of positive clones ... |
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| Keywords: | Cattle Theileria sergenti 18S rRNA gene Cloning Molecular taxonomy |
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