Genotyping of isolates ofTaylorella equigenitalis from thoroughbred brood mares in Japan |
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| Authors: | T. Miyazawa M. Matsuda Y. Isayama T. Samata Y. Ishida S. Ogawa K. Takei M. Honda M. Kamada |
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| Affiliation: | (1) Laboratory of Molecular Biology, Department of Biosciences College of Environmental Health, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara;(2) Department of Immunology College of Environmental Health, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara;(3) Department of Hygiene, College of Environmental Health, Azabu University, 1-17-71 Funchinobe, 229 Sagamihara;(4) Epizotic Research Station, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Kokubunji-machi, 329-04 Tochigi, Japan |
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| Abstract: | Profiles of the genomic DNA of 104 strains ofT. equigenitalis isolated from brood mares with contagious equine metritis in Hokkaido during the breeding seasons from 1980 to 1993, as well as those of five strains (SS28, EQ56, EQ59, EQ70 and HH139) previously isolated in Japan were examined after restriction digestion and crossed-field gel electrophoresis. These profiles were essentially identical to each other and the various isolates and strains appeared to have a common genotype, designated  genotype J , with respect to two restriction enzymes,ApaI andNotI. These results suggest a common source for all these isolates obtained over the course of more than 10 years in Japan.Abbreviations CEM contagious equine metritis - CFGE crossed-field gel electrophoresis - PFGE pulsed-field gel electrophoresis |
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| Keywords: | contagious equine metritis DNA genome pulsed-field gel electrophoresis strain identification Taylorella equigenitalis |
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