甘草种衣剂对甘草生长和甘草酸含量的影响

目的了解甘草种衣剂对一年生甘草中根长、根干重和甘草酸含量的影响,为甘草包衣技术的推广提供理论依据和技术支撑。方法采用刻度尺直接测量根长,烘干后测量根干重的方法,甘草酸含量测定采用高效液相色谱法,对甘草根部粉末的70%乙醇提取物进行分析。以十八烷基硅烷键合硅胶为填充剂,乙腈为流动相A,0.05%磷酸溶液为流动相B,进行梯度洗脱,检测波长为237nm。结果甘草种衣剂可以增加一年生甘草根长、根干重和甘草酸含量,包衣组甘草中的甘草酸含量比未包衣组的增加了0.01%。结论甘草种衣剂可以促进增加甘草根长和根干重,进而促进甘草根部的生长,HPLC法方法准确,灵敏度高,重复性好,成本低,可用于甘草中甘草酸含量测定,甘草种衣剂可以提高甘草中甘草酸含量,进而提高了甘草的品质。

Effects of seed coating agent on the growth and content of glycyrrhizic acid of Glycyrrhiza uralensis

Objective To understand Effects of seed coating agent on the growth and content of glycyrrhizic acid of Glycyrrhiza uralensis, providing theoretical basis and technical support for the promotion of seed coating technology. Method Analysis the 70% ethanol extract of flour of G. uralensis root by measuring the length of G. uralensis root with dividing ruler, measuring the weight of G. uralensis after drying, and measuring the content of glycyrrhizic acid using HPLC. Elution by using octadecyl bonded silica as filler, acetonitrile as mobile phase A, 0.05% phosphoric acid solution as mobile phase B, the detection wavelength was 237nm.Result Glyeyrrhizae seed dressing can incresase the length and weight of Glycyrrhizae root and content of Glycyrrhizic acid, the coating group, the content of glycyrrhizic acid in the coated group increased by 0.01% than that of the uncoated group. Conclusion Seed coating agent can help increasing length and weight of root, and promoting the growth of G. uralensis. HPLC is an accurate method with high sensitivity good repeatability and low cost, it can be used for determination of glycyrrhizic acid in G. uralensis. Seed coating agent can improve the content of Glycyrrhizic acid in G. uralensis.