| 猪OGP蛋白基因克隆及分泌载体的构建与鉴定 |
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| 引用本文： | 李方正. 猪OGP蛋白基因克隆及分泌载体的构建与鉴定[J]. 中国农学通报, 2012, 28(29): 83-86 |
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| 作者姓名： | 李方正 |
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| 作者单位： | 青岛农业大学动物科技学院,山东青岛,266109 |
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| 基金项目： | 青岛农业大学高层次人才科研基金资助“雌二醇对输卵管上皮细胞钙网蛋白表达的影响及调控的信号途径”(631209) |
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| 摘 要： | 为了获得重组猪OGP蛋白，构建OGP分泌表达载体，以使猪输卵管特异性糖蛋白在体外受精中发挥重要作用。从猪输卵管中提取总RNA，运用RT-PCR技术扩增猪OGP全部编码序列，克隆到pET22b载体中，构建成pET22b-OGP分泌表达载体，对其进行酶切、PCR和测序鉴定。结果表明：成功构建了OGP分泌表达载体，为进一步研究该OGP在猪体外受精中的功能奠定了基础。
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| 关 键 词： | 存活率 存活率 |
| 收稿时间： | 2012-05-08 |
| 修稿时间： | 2012-06-21 |
Clone and Construction of Secretory Expression Vector of Porcine OGP |
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| Li Fangzheng. Clone and Construction of Secretory Expression Vector of Porcine OGP[J]. Chinese Agricultural Science Bulletin, 2012, 28(29): 83-86 |
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| Authors: | Li Fangzheng |
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| Affiliation: | Li Fangzheng(College of Animal Science and Technology,Qingdao Agriculture University,Qingdao Shandong 266109) |
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| Abstract: | The OGP protein was important in the in vitro fertilization of pig.In order to produce the recombination OGP protein,the OGP secretory expression vector was constructed.The total RNA was extracted from porcine oviduct and coding sequence of porcine OGP was amplified using RT-PCR method.The OGP CDS was cloned into the pET22b vector and then identified by restriction enzyme analysis,PCR amplification and DNA sequencing.The results showed that the pET22b-OGP vector was constructed successfully,which provided advantage for further study of OGP function at the porcine in vitro fertilization. |
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| Keywords: | Porcine OGP clone secretory expression |
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