现行牛IVF程序在水牛体外受精的应用研究

本研究对我所现行的牛体外受精程序 ( IVF)应用于水牛体外受精的可行性进行了探讨。结果表明 ,水牛卵巢生长卵泡少 ,平均仅可回收 5枚卵母细胞 ,只相当于黄牛 ( 14.3个 )的1/ 3。水牛卵母细胞的体外成熟率为 51.7% ,体外受精卵裂率为 58.7% ,均极显著地低于黄牛(分别为 87.3%和 69.6% ,P<0 .0 1)。水牛卵母细胞经体外受精后 ,早期胚胎的体外发育速度较黄牛快 ,体外受精后的第 6d囊胚发育率占 36.4 % ,累计到第 7d时占 80 .7% ,极显著地高于黄牛 ( 57.6% ,P<0 .0 0 1)。水牛早期胚胎的体外囊胚发育率为 31.5% ,与黄牛的 ( 39.2 % )差异不显著 ;孵化囊胚率为 73.8% ,也与黄牛的相仿。实验结果表明 ,我所现行的牛 IVF程序应用于水牛的体外受精研究是有效、可行的 ,特别是早期胚胎的体外培养系统 ,已接近于黄牛的水平。但尚需设法提高水牛卵母细胞的体外成熟培养效果 ,以提高水牛体外受精的整体效率

Effectof Bovine IVF Procedure on Buffalo IVF

The aim of this study was to elucidate the posibility of appling bovine IVF procedure on buffalo IVF.Aspiration of873buffalo ovaries has provided an average of5 oocytes per ovary,which is about1 /3obtained from bovine ovary.The maturation rate (5 1 .7% ) and cleavage rate (5 8.7% ) of buffalo oocytes were significantly lower than those of bovine oocytes(87.3% ,6 9.9% respectively,P<0 .0 1 ) .The rate at which early buffalo embryos developed to blastocyst stage was faster than that of bovine embryos.The blastocyst rate which developed from cleavaged embryos wassimilar between buffalo and cattle(31 .5 % and 39.2 % ,respectively) ,so was the hatched blastocystrate.The resultsindicate thatitis effective using our bovine IVF procedure in buffalo oocytes IVF.