| 基于性状和分子标记的小麦抗旱近等基因系的分离 |
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| 引用本文： | 董建力,白海波,朱永兴,吕学莲,王 娜,李树华. 基于性状和分子标记的小麦抗旱近等基因系的分离[J]. 中国农学通报, 2016, 32(12): 33-37. DOI: 10.11924/j.issn.1000-6850.casb15070184 |
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| 作者姓名： | 董建力 白海波 朱永兴 吕学莲 王 娜 李树华 |
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| 作者单位： | (;1.宁夏农业生物技术重点实验室,银川 750002；;2.宁夏医科大学,银川 750002） |
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| 基金项目： | 国家自然科学基金“小麦碳同位素分辨率QTL定位及其与环境互作效应研究”(31160285)；宁夏回族自治区农业育种专项“小麦分子育种技术应用研究”(2013NYYZ02)；宁夏医科大学项目“水分胁迫对春小麦碳同位素分辨率等指标的影响及相关性研究”(201201)；宁夏农林科学院先导资金项目“春小麦灌浆期耐热相关基因的挖掘”(NKY3-15-07)。 |
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| 摘 要： | 创建小麦‘宁春4号’抗旱近等基因系，为其在生产中的应用和抗旱遗传改良提供有利用价值的材料。利用重组自交系(RILs)合适世代(F6或F7)的单株具有一定杂合率的特点，采用性状和分子标记分离近等基因系的方法，在‘宁春4号’/‘宁春27号’RILs一个家系RIL-97内分离出‘宁春4号’抗旱近等基因系，经抗旱性及特征特性鉴定表明，该近等基因系抗旱性较强，其他形态特征特性与‘宁春4号’基本一致。这些近等基因系可进一步用来精确定位抗旱相关性状QTL位点。利用RILs群体合适世代结合分子标记是快速构建近等基因系的有效方法。
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| 关 键 词： | 草莓 草莓 SRAP 体系优化 引物筛选 |
| 收稿时间： | 2015-07-31 |
| 修稿时间： | 2016-04-13 |
Segregation of Near-Isogenic Line of Wheat with Drought Resistance Based on Traits and Molecular Marker |
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| Dong Jianli,Bai Haibo,Zhu Yongxing,Lv Xuelian,Wang Na and Li Shuhua. Segregation of Near-Isogenic Line of Wheat with Drought Resistance Based on Traits and Molecular Marker[J]. Chinese Agricultural Science Bulletin, 2016, 32(12): 33-37. DOI: 10.11924/j.issn.1000-6850.casb15070184 |
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| Authors: | Dong Jianli Bai Haibo Zhu Yongxing Lv Xuelian Wang Na Li Shuhua |
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| Affiliation: | (;1.Ningxia Agricultural Bio-Technological Laboratory, Yinchuan 750002;2.Ningxia Medical University, Yinchuan 750002) |
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| Abstract: | The study aims to create the drought resistance near-isogenic lines (NILs) of wheat ‘Ningchun 4’, and provide valuable materials for the NILs application in production and the genetic improvement of drought resistance. Due to the certain heterozygous ratio existing in proper generation (F6 or F7) of the recombinant inbred lines (RILs), NILs separation method was applied based on the traits and the molecular markers. The NILs of ‘Ningchun4’ plants with drought were isolated from RIL-97 of ‘Ningchun4’בNingchun27’, the identification of drought resistance and characteristics indicated that the NILs had strong drought resistance, and other morphological characteristics were basically identical to ‘Ningchun4’. The NILs would be used for the accurate positioning of the drought QTLs. The method based on RILs molecular markers of the proper generation of the recombinant was an effective way to construct NILs rapidly. |
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| Keywords: | wheat recombinant-inbred line near-isogenic line drought resistance |
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