披碱草和野大麦及其杂种F1与BC1酯酶同工酶分析

采用聚丙烯酰胺垂直平板电泳技术对披碱草和野大麦及其杂种F₁与回交1代酯酶同工酶进行比较分析。结果表明,亲本披碱草和野大麦有位点相同的9条基带,从酶蛋白分子水平验证,两亲本有较近的亲缘关系。杂种F₁的酯酶同工酶谱主要表现为双亲酶带的互补类型,亲本的个别酶带在杂种F₁有丢失现象,正反交杂种F₁均产生一条杂种带。杂种F₁酶谱有偏向母本遗传的倾向。正反交的回交1低酯酶同工酶谱基本相同,但也存在一定的差异,回交1代的酯酶同工酶谱明显地偏向轮回亲本野大麦。酯酶同工酶具有多态性,可作为遗传标记用于杂种鉴定和回交后代目标性状植株的检测。

Analysis of Esterase Isozymes of Elymus dahuricus,Hordeum brevisubulatum Their Hybrid F1 and BC1

The esterase(EST)isozymes of Elymus dahuricus,Hordeum brevisubulatum,their Hybrid F₁ and BC₁ were analyzed by polyacrylamid gel electrophoresis.The results showed that toth E.dahuricus and H.brevisubulatum had 9 base bands at same location,whhich meant that the relationship between E.dahuricus and H.brevisubulatum was closer;the zymogram types of the hybrid F₁ were mainly the mutual suplementary isozymes of the parents,very few isozyme bands of the parents were lost,the hybrid F₁ had a hybrid band,the zymograms of the hybrid F₁ showed a tendency toward the maternal type;there were no significant differences in EST zymograms between H.brevisubulatum×E.dahuricus×H.brevisubulatum(BC₁)and E.dahuricus×H.brevisubulatum×H.brevisubulatum(BC₁),but some differences existed,the zymograms of BC₁ showed a tendency toward the recurrent parent H.brevisublatum obviously.There were polymorphisms in isozymes among the parents,their hybrid F₁ and BC₁,which can be used as genetic marker to identify hybrid and determine the plants with object characteristics in backcross desendants.