Microwave-assisted rapid determination of vitamins a and e in beverages |
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Authors: | Höller Ulrich Wolter Didier Hofmann Peter Spitzer Volker |
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Institution: | Human Nutrition and Health, Analytics (VFHA), Roche Vitamins Ltd., CH-4070 Basel, Switzerland. |
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Abstract: | A new rapid procedure for the determination of vitamins A and E in beverages has been developed and validated. Key steps include a microwave-assisted saponification of the sample and a single-step extraction of the vitamins prior to HPLC analysis. All sample preparation steps are carried out consecutively in the same vial. The vitamins are determined using normal-phase (Si-60) HPLC with fluorescence detection. The method is applicable to beverages with a content of all-trans-retinol >0.14 mg/L and/or a content of alpha-tocopherol >1 mg/L. Recoveries determined by spiking experiments ranged from 91.3 to 106.3%. The precision of the method is characterized by relative standard deviations of <2% for alpha-tocopherol and <5% for all-trans-retinol. |
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