| 荧光蛋白标记表达H6亚型禽流感病毒血凝素基因 |
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| 引用本文： | 华文君,乔宪凤,刘西梅,郑新民,周荆荣.荧光蛋白标记表达H6亚型禽流感病毒血凝素基因[J].湖北农业科学,2007,46(4):513-516. |
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| 作者姓名： | 华文君 乔宪凤 刘西梅 郑新民 周荆荣 |
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| 作者单位： | 湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉,430064;湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉,430064;湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉,430064;湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉,430064;湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉,430064 |
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| 基金项目： | 湖北省科技攻关项目
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湖北省重点实验室开放课题 |
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| 摘 要： | 研究构建了H6N2亚型AIV的核酸疫苗表达载体,以绿色荧光蛋白基因作为报告基因,构建了HA与报告基因的融合基因,并克隆于pAVX1表达载体,再经脂质体转染BHK细胞进行体外表达试验.构建的表达载体pAVX1-H6A-GFP酶切鉴定已克隆上融合基因;体外表达试验中,脂质体转染24 h后出现微弱荧光,48 h后见较强的荧光表达,从而成功实现了用荧光蛋白标记表达了H6亚型禽流感病毒血凝素基因.
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| 关 键 词： | 禽流感病毒 HA基因 表达 |
| 文章编号： | 0439-8114（2007）04-0513-04 |
| 修稿时间： | 2007-04-13 |
Expressing of HA Gene of H6 Subtype Avian Influenza Virus with Fluorescent Protein Marked |
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| HUA Wen-jun,QIAO Xian-feng,LIU Xi-mei,ZHENG Xin-ming,ZHOU Jing-rong.Expressing of HA Gene of H6 Subtype Avian Influenza Virus with Fluorescent Protein Marked[J].Hubei Agricultural Sciences,2007,46(4):513-516. |
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| Authors: | HUA Wen-jun QIAO Xian-feng LIU Xi-mei ZHENG Xin-ming ZHOU Jing-rong |
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| Institution: | Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Science, Wuhan 430064,China |
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| Abstract: | H6N2 subtype AIV DNA vaccine expression vector was constructed in this research,the vaccine was marked with green fluorescent protein. The HA-GFP fusion gene was cloned on plasmid pAVX1 expression vector,the vaccine was expressed in BHK cell by lipofection .The expression vector pAVX1-H6A-GFP succeeded to clone the fusion gene HA-GFP by enzymolysis identification. In vitro expression tests, the cells got weak fluorescence after lipofection 24 hours,and got strong fluorescence after lipofection 48 hours. This indicated the research succeeded to express HA gene of H6 subtype Avian influenza virus of HA gene with green fluorescent protein marked. |
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| Keywords: | avian influenza virus hemagglutinin(HA) gene express |
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