| 鱼糜制品中基因组DNA提取方法的比较 |
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| 引用本文： | 陈赟,汪之和.鱼糜制品中基因组DNA提取方法的比较[J].湖北农业科学,2007,46(4):520-522. |
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| 作者姓名： | 陈赟 汪之和 |
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| 作者单位： | 上海水产大学食品学院,上海,杨浦,200090 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 将分别采用普通酚-氯仿抽提法和试剂盒(柱吸附法)提取DNA的两种方法加以比较,以确定最适提取鱼糜制品DNA的方法.将鱼肉和鱼糜制品作为原料,用两种方法提取基因组DNA,利用分光光度计测定其A260与A280的吸光值,计算比率估计核酸的纯度,并利用琼脂糖凝胶电泳观察DNA片段在凝胶中的位置;并用线粒体16S rRNA基因PCR扩增产物鉴定所提取的基因组DNA.
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| 关 键 词： | 基因组DNA OD值 凝胶电泳 PCR |
| 文章编号： | 0439-8114（2007）04-0520-03 |
| 修稿时间： | 2007-02-16 |
The Comparison of the Methods of Genomic DNA Extracting from Surimi Products |
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| CHEN Yun,WANG Zhi-he.The Comparison of the Methods of Genomic DNA Extracting from Surimi Products[J].Hubei Agricultural Sciences,2007,46(4):520-522. |
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| Authors: | CHEN Yun WANG Zhi-he |
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| Institution: | Food College of Shanghai Fisheries University,Yangpu 200090,Shanghai,China |
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| Abstract: | The method of phenol-chloroform extraction and Kit(column) was compared to determine the optimal method to extract DNA from surimi products.The muscle of fish and surimi products would be as raw materials.It was inspected under spectrophotometer to get absorption value at 260nm and 280nm and estimated the rate of nucleic acid purity,observed the size of DNA fragments in agarose gel electrophoresis;identified the genomic DNA extracted using mitochondrial 16S rRNA gene PCR products. |
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| Keywords: | genomic DNA the value of OD gel electrophoresis PCR |
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