| 仿刺参体腔细胞CD35的化学发光免疫检测 |
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| 引用本文： | 张峰,张莉,倪慧,张斯.仿刺参体腔细胞CD35的化学发光免疫检测[J].大连水产学院学报,2011,26(2):176-179. |
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| 作者姓名： | 张峰 张莉 倪慧 张斯 |
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| 作者单位： | 大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室;大连海洋大学生命科学与技术学院; |
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| 基金项目： | 国家自然科学基金资助项目(3047132) |
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| 摘 要： | 应用化学发光免疫检测（Chemiluminesent Immunoassay,CLIA）技术检测仿刺参Apostichopus japoni-cus体腔细胞CD35。单克隆CD35一抗抗体,用辣根过氧化物酶标记羊抗鼠IgG二抗,选择对碘苯酚作为发光增强剂,鲁米诺和过氧化氢作为辣根过氧化物酶的底物;对照组用无菌海水代替一抗和二抗,进行化学发光免疫检测。结果显示：试验组发光值（1 321 596.00）和对照组（16 773.20）的发光值差异极显著（P〈0.01）;仿刺参体腔细胞膜上能够检测到CD35的存在,表明仿刺参体腔细胞膜上具有补体受体。
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| 关 键 词： | 化学发光免疫检测 仿刺参 体腔细胞 CD35 |
Detection of CD35 in coelomocytes of sea cucumber Apostichopus japonicus by chemiluminescence immunoassay |
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| ZHANG Feng,ZHANG Li,NI Hui,ZHANG Si.Detection of CD35 in coelomocytes of sea cucumber Apostichopus japonicus by chemiluminescence immunoassay[J].Journal of Dalian Fisheries University,2011,26(2):176-179. |
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| Authors: | ZHANG Feng ZHANG Li NI Hui ZHANG Si |
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| Institution: | ZHANG Feng1,2,ZHANG Li1,NI Hui1,ZHANG Si1,(1.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean University,Dalian 116023,China,2.School of Life Science and Technology,China) |
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| Abstract: | The CD35 analogue in coelomocytes of sea cucumber Apostichopus japonica was defected by chemiluminesent immunoassay(CLIA) in which monoclonal CD35 antibody was used as primary antibody,horseradish peroxidase(HRP)-conjugated goat monoclonal anti-mouse IgG as secondary antibody,p-iodo-phenol as luminous reinforcing agent,and luminol solution-H2O2 as the substrate of HRP.In the control group,free bacteria physiological solution as monoclonal anti-secondary antibody.There was significant difference in the relat... |
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| Keywords: | chemiluminescence immunoassay Apostichopus japonicus coelomocyte CD35 |
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