| 蝴蝶兰愈伤组织诱导和体细胞胚胎发生的研究 |
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| 引用本文： | 吕晓辉,黄象男,郭晓慧,宿显瑞,臧新. 蝴蝶兰愈伤组织诱导和体细胞胚胎发生的研究[J]. 安徽农业科学, 2007, 35(25): 8068-8070 |
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| 作者姓名： | 吕晓辉 黄象男 郭晓慧 宿显瑞 臧新 |
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| 作者单位： | 郑州大学生物工程系,河南郑州,450001;郑州大学生物工程系,河南郑州,450001;郑州大学生物工程系,河南郑州,450001;郑州大学生物工程系,河南郑州,450001;郑州大学生物工程系,河南郑州,450001 |
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| 摘 要： | 研究了蝴蝶兰愈伤组织的诱导和通过体细胞胚胎发生的植株再生。原球茎在含有蔗糖的VW培养基上形成愈伤组织。结果表明,最有效的诱导愈伤组织的培养基为VW+0.1 mg/L BA+0.1 mg/L 2,4-D+60 g/L蔗糖或者VW+0.3 mg/L BA+0.1 mg/L NAA+40 g/L蔗糖。愈伤组织在被转到不含植物激素的VW培养基上易形成原球茎。组织学观察证实了体细胞胚胎结构的形成,暗示了试验中诱导的愈伤组织即为体细胞胚。
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| 关 键 词： | 蝴蝶兰 愈伤组织 原球茎 体细胞胚胎发生 |
| 文章编号： | 0517-6611（2007）25-08068-03 |
| 修稿时间： | 2007-05-16 |
Callus Induction and Somatic Embryogenesis of Phalaenopsis |
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| LV Xiao-hui et al. Callus Induction and Somatic Embryogenesis of Phalaenopsis[J]. Journal of Anhui Agricultural Sciences, 2007, 35(25): 8068-8070 |
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| Authors: | LV Xiao-hui et al |
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| Affiliation: | Bioengineering Department,Zhengzhou University,Zhengzhou,Henan 450001 |
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| Abstract: | Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis were researched.Protocorm-like body(PLB)segments formed callus on VW medium with sucrose.The results showed that the medium containing 0.1 mg/L BA,0.1 mg/L 2,4-D and 60 g/L sucrose or 0.3 mg/L BA,0.1 mg/L NAA and 40 g/L sucrose was effective for callus induction.The callus easily formed PLBs after being transferred to VW medium without plant hormone.Histological observation proved that the somatic embryo structure formation and suggested that the callus induced in this work was somatic embryos. |
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| Keywords: | Phalaenopsis Callus Protocorm-like body(PLB) Somatic embryogenesis |
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