| 陕北白绒山羊Lhx2基因cDNA序列的克隆与原核表达 |
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| 引用本文： | 刘 敏,白丁平,耿荣庆,方 堃,陈玉林. 陕北白绒山羊Lhx2基因cDNA序列的克隆与原核表达[J]. 西北农业学报, 2012, 21(4): 1-5 |
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| 作者姓名： | 刘 敏 白丁平 耿荣庆 方 堃 陈玉林 |
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| 作者单位： | 西北农林科技大学 动物科技学院,陕西 杨凌,712100 |
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| 基金项目： | 农业部转基因生物新品种培育科技重大专项，国家自然科学基金 |
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| 摘 要： | 根据GenBank中已发表的Lhx2基因序列设计引物,采用RT-PCR方法,从陕北白绒山羊皮肤组织克隆Lhx2基因编码区cDNA,对其进行生物信息学分析,并构建原核表达载体pET-Lhx2,在大肠杆菌BL21(DE3)中表达。最终获得陕北白绒山羊Lhx2基因,长1 221bp;酶切和测序结果显示,原核表达载体pET-Lhx2构建成功;SDS-PAGE和Western blot分析表明,重组原核表达载体在大肠杆菌中成功表达出目的融合蛋白。
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| 关 键 词： | 陕北白绒山羊 Lhx2 克隆 原核表达 |
Cloning and Prokaryotic Expression of Lhx2 Gene from Shaanbei White Cashmere Goat |
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| LIU Min,BAI Dingping,GENG Rongqing,FANG Kun and CHEN Yulin. Cloning and Prokaryotic Expression of Lhx2 Gene from Shaanbei White Cashmere Goat[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(4): 1-5 |
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| Authors: | LIU Min BAI Dingping GENG Rongqing FANG Kun CHEN Yulin |
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| Affiliation: | (College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | The cDNA of Lhx2 gene was amplified from the skin of Shaanbei White Cashmere Goat by RT-PCR using one pair of primers which was designed and synthesized according to the Lhx2 gene sequence in GenBank.The biological information analysis was performed with it.The prokaryotic expression vector pET-Lhx2 was constructed and expressed in E.coli BL21(DE3).The cDNA of Shaanbei White Cashmere Goat Lhx2 gene was 1 221 bp.Restriction enzyme mapping and sequencing showed that prokaryotic expression vector was constructed successfully.SDS-PAGE and Western blot showed that the recombinant prokaryotic expression vector expressed the fusion protein(65 ku)successfully. |
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| Keywords: | Shaanbei White Cashmere Goats Lhx2 Cloning Prokaryotic expression |
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