| 普通小麦离体胚组织的脱分化和再分化培养特性 |
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| 引用本文： | 杨亚辉,陈耀锋,李春莲,薛 芳,安 强,周文丽. 普通小麦离体胚组织的脱分化和再分化培养特性[J]. 西北农业学报, 2012, 21(2): 26-30 |
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| 作者姓名： | 杨亚辉 陈耀锋 李春莲 薛 芳 安 强 周文丽 |
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| 作者单位： | 西北农林科技大学农学院,陕西杨凌,712100 |
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| 基金项目： | 国家转基因生物新品种培育项目，陕西省13115重大科技专项，唐仲英育种项目，陕西省科技攻关项目 |
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| 摘 要： | 以西农1376和小偃22两个普通小麦品种为材料,对授粉后不同时间段幼胚进行脱分化、再分化培养,分析不同胚龄的小麦离体胚培养特性。结果表明,胚龄22d时,西农1376和小偃22胚诱导形成的愈伤组织频率最高,胚龄26d胚发芽率最高,胚龄16d愈伤组织再分化率最高。在小麦离体胚组织培养中,胚龄是影响小麦胚离体培养特性的关键因素。
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| 关 键 词： | 胚龄 脱分化 再分化 |
Studies on Dedifferentiation and Re differentiation Characteristics of in vitro Embryos of Common Wheat |
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| YANG Yahui,CHEN Yaofeng,LI Chunlian,XUE Fang,AN Qiang and ZHOU Wenli. Studies on Dedifferentiation and Re differentiation Characteristics of in vitro Embryos of Common Wheat[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(2): 26-30 |
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| Authors: | YANG Yahui CHEN Yaofeng LI Chunlian XUE Fang AN Qiang ZHOU Wenli |
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| Affiliation: | (College of Agronomy,Northwest A&F University,Yangling Shaanxi 72100,China) |
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| Abstract: | The in vitro culture characteristics of wheat immature embryos in different developmental stages were systematically studied through the dedifferentiation and re-differentiation.Immature embryos of different developmental stages of two wheat cultivars,Xinong 1376 and Xiaoyan 22 were used as the materials,the culture characteristics were statistically analyzed through dedifferentiation and re-differentiation.The results showed that the callus induction frequencies of Xinong 1376 and Xiaoyan 22 were the highest for the embryos of 22 days after pollination;for the embryos of 26 days,the germination rate was the highest;for the 16 days,the callus re-differentiation was the highest.The age of embryo was the key factor which affected the in vitro embryo culture characteristics of wheat. |
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| Keywords: | Embryo age Dedifferentiation Re-differentiation |
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