普通小麦离体胚组织的脱分化和再分化培养特性

以西农1376和小偃22两个普通小麦品种为材料,对授粉后不同时间段幼胚进行脱分化、再分化培养,分析不同胚龄的小麦离体胚培养特性。结果表明,胚龄22d时,西农1376和小偃22胚诱导形成的愈伤组织频率最高,胚龄26d胚发芽率最高,胚龄16d愈伤组织再分化率最高。在小麦离体胚组织培养中,胚龄是影响小麦胚离体培养特性的关键因素。

Studies on Dedifferentiation and Re differentiation Characteristics of in vitro Embryos of Common Wheat

The in vitro culture characteristics of wheat immature embryos in different developmental stages were systematically studied through the dedifferentiation and re-differentiation.Immature embryos of different developmental stages of two wheat cultivars,Xinong 1376 and Xiaoyan 22 were used as the materials,the culture characteristics were statistically analyzed through dedifferentiation and re-differentiation.The results showed that the callus induction frequencies of Xinong 1376 and Xiaoyan 22 were the highest for the embryos of 22 days after pollination;for the embryos of 26 days,the germination rate was the highest;for the 16 days,the callus re-differentiation was the highest.The age of embryo was the key factor which affected the in vitro embryo culture characteristics of wheat.