3种菌株产木聚糖酶同工酶及其酶解产物的比较

探讨3种菌株所产木聚糖酶组分及其酶解产物的差异,为酶制剂和功能低聚木糖的研发和应用提供理论依据。采用SDS-PAGE电泳,研究黑曲霉C71、康宁木霉M46及XYNB重组工程菌G81所产的木聚糖酶同工酶。结果表明,C71、M46及G81分别产3种、4种和2种木聚糖酶同工酶;以桦木木聚糖为底物,在酶添加量为70U/g、底物20g/L、反应时间10h条件下进行酶解,采用TLC及HPLC分析酶解产物。TLC结果表明,C71和M46酶解液中含有木糖、木二糖和木三糖;G81酶解液中含有木二糖、木三糖和木四糖。HPLC结果表明,C71酶解液中木糖、木二糖和木三糖的质量浓度分别为2.4、1.3和1.7g/L;M46酶解液中木糖、木二糖和木三糖的质量浓度分别为1.5、1.8和2.1g/L;G81酶解液中木二糖、木三糖和木四糖的质量浓度分别为2.6、2.7和1.4g/L。不同菌株所产木聚糖酶组分及其酶解产物存在差异,低聚木糖的研发应根据酶学特性,选择不同的单一或复合酶制剂。

Comparative Study on Xylanase Isozymes and Their Hydrolysates from Three Different Strains

Xylanases were produced by Aspergillus niger C71,Trichoderma koningii M46 and recombinant XYNB C81.These xylanase isozymes were analyzed with SDS-PAGE electrophoresis.The hydrolyzing conditions of xylanases were as follow: substrate is birchwood,dosage of enzyme is 70 U/g,substrate concentration is 20 g/L,the hydrolysis time is 10 h.The hydrolysates of xylanases from three different strains were detected by using TLC and HPLC.The results showed that the components of xylanase were N-1,N-2,N-3(produced by Aspergillus niger G71);Y-1,Y-2,Y-3,Y-4(produced by Trichoderma koningii M46) and Z-1,Z-2(produced by the recombinant XYNB G81).TLC and HPLC analysis indicated that the hydrolysates of xylanases produced by A.niger C71 and Trichoderma reesi M46 contained xylose(2.4 g/L,1.5 g/L,respectively),xylobiose(1.3 g/L,1.8 g/L,respectively) and xylotriose(1.7 g/L,2.1 g/L,respectively).The hydrolysates of xylanase produced by XYNB G81 contained xylobiose(2.6 g/L),xylotriose(2.7 g/L) and xylotetraose(1.4 g/L).The experimental results provided theoretical basis for the development of XOS.