鄂西北产猫眼草提取物体外抗氧化性及光稳定性测试

猫眼草为鄂西北地区常见的野生有毒植物种,经测定具有较强的杀虫、抑菌活性。为了深入开发此植物资源,室内测定其提取物的体外抗氧化性及光稳定性。采用α-脱氧核糖法、邻苯三酚自氧化法、DPPH自由基清除法和铁氰化钾还原法等方法体外评价猫眼草提取物的抗氧化性。同时,采用高效液相色谱法测定其光稳定性。结果表明:①随着猫眼草提取物质量浓度的升高,羟自由基、氧自由基、DPPH自由基的清除率上升,分别高达82.04%、87.99%、87.73%。在一定范围内,随着猫眼草提取物质量浓度的升高,对铁氰化钾的还原能力也在升高。②猫眼草提取物的光稳定性较差,Tween-20(表面活性剂)和水杨酸苯酯(光稳定剂)对其具有一定的光稳定增效作用。

In vitro Antioxidant and Photostability Testing for Extracts of Enphorbia lunulata Bgel from Northwestern Hubei

Enphorbia lunulata Bgel,a common toxic plant species distributed in Northwestern Hubei,was detected to have good insecticidal and fungicidal activities.To exploit this resource,it’s neccessary to evaluate in vitro antioxidant and photostability of its extract.The following methods was used to measure the antioxidantion of E.lunulata extracts including a-deoxyribose method,adjacent pyrogallol auto-oxidation method,DPPH radical scavenging method and iron potassium ferricyanide reduction method and its photostability was tested by HPLC method as well.The results showed as followed:(i) With the concentration increase of the E.lunulata extracts,the clearance rate of the hydroxyl radicals,the oxygen free radicals,and DPPH free radicals rises up,reaching up to 87.73%,87.99%,and 82.04%,respectively.Within a certain range,along with the concentrations of E.lunulata extracts,the iron potassium cyanide reductive ability is also on the rise.(ii) The testing revealed that E.lunulata extracts had bad photostability and the Tween-20(surfactant) and phenyl salicylate(light stabilizer) were found to have a certain degree of light atability on E.lunulata extracts.