| 巴西橡胶树LIM结构域基因克隆与生物信息学分析 |
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| 引用本文： | 吴瑞,朱家红,张全琪,张治礼.巴西橡胶树LIM结构域基因克隆与生物信息学分析[J].热带生物学报,2010,1(1):62-66. |
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| 作者姓名： | 吴瑞 朱家红 张全琪 张治礼 |
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| 作者单位： | 吴瑞(海南大学,农学院,海南,海口,570228;中国热带农业科学院,热带作物生物技术研究所,农业部热带生物技术重点开放实验室,海南,海口,571101);朱家红,张全琪(中国热带农业科学院,热带作物生物技术研究所,农业部热带生物技术重点开放实验室,海南,海口,571101);张治礼(中国热带农业科学院,热带作物生物技术研究所,农业部热带生物技术重点开放实验室,海南,海口,571101;海南省农业科学院,海南,海口,571000) |
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| 基金项目： | 国家自然科学基金，中央级公益性科研院所业务费专项资金 |
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| 摘 要： | 根据乙烯利刺激巴西橡胶树(Hevea brasiliensis)胶乳差异表达cDNA文库的EST序列信息,利用RACE技术从胶乳中成功克隆了1个含有LIM结构域的未知功能新基因HbLIM的全长cDNA。生物信息学分析表明,该基因全长cDNA由1016bp的碱基组成,拥有1个570bp的开放阅读框编码189个氨基酸残基;推测氨基酸序列富含Lys,Ser,Ala和Leu,含有2个LIM结构域和2个N-豆蔻酰化位置。序列相似性分析表明,HbLIM编码的氨基酸序列与蓖麻、杨毛、葡萄相应的LIM基因的相似性高达98%,95%和92%。这些研究结果为深入探讨LIM结构域蛋白在巴西橡胶树乳管发育及乳管代谢中的功能奠定了基础。
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| 关 键 词： | 巴西橡胶树 基因克隆 LIM基因 生物信息学 |
Cloning and Bioinformatics Analysis of a LIM Domain Protein Gene from Hevea brasiliensis |
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| WU Rui,ZHU Jia-hong,ZHANG Quan-qi,ZHANG Zhi-li.Cloning and Bioinformatics Analysis of a LIM Domain Protein Gene from Hevea brasiliensis[J].Journal of Tropical Biology,2010,1(1):62-66. |
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| Authors: | WU Rui ZHU Jia-hong ZHANG Quan-qi ZHANG Zhi-li |
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| Institution: | 1. College of Agriculture, Hainan University, Haikou 570228, China; 2. Key Laboratory of Ministry of Agriculture for Tropical Crop Biotechnology, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 3. Hainan Academy of Agricultural Sciences, Haikou 571100; China) |
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| Abstract: | Based on the information of EST sequences from latex SSH cDNA library stimulated by ethephon, RACE was used to isolate the full length eDNA of an unknown functional novel gene with two LIM domains from H. brasiliensis, HbLIM. Bioinformatics analysis results showed which comprises of 1 016bp with a 570bp ORF and encodes a 189 animo acid residues. The deduced animo acid sequence with two LIM domains and two Nmyristoylation sites is rich in Lys,Ser,Ala and Leu and has high identity of 98%, 95% and 92% to the homologues from Pollen specific, Populus trichocarpa and Vitis vinifera. These results were very important for elucidation of the （unctions of LIM-domain protein in latercifer development and metabolism in It. brasiliensis. |
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| Keywords: | Hevea brasiliensis Gene cloning LIM gene bioinformatical analysis |
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