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Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.)
Authors:Patricia Roeckel-Drevet  Geneviève Gagne  Said Mouzeyar  Laurent Gentzbittel  Jacqueline Philippon  Paul Nicolas  Denis Tourvieille De Labrouhe  Felicity Vear
Institution:(1) Unité Associée Université Blaise Pascal-I.N.R.A., Organisation et Variabilité des Génomes Végétaux', 4 rue Ledru, 63038 Clermont Ferrand cedex 1, France;(2) Station d'Amélioration des Plantes, I.N.R.A., Domaine de Crouelle, 63039 Clermont-Ferrand cedex 2, France;(3) G.I.E. Cartisol, 4 rue Ledru, 63038 Clermont-Ferrand cedex 1, France
Abstract:Summary The Pl6 locus in the inbred sunflower (Helianthus annuus L.) line HA335 giving resistance to French races of downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni. was localized by molecular techniques. A bulked segregant analysis was made on the F2 progeny from a cross between this line and H52, a downy mildew susceptible line. The resistance gene in HA335 was found to have the same linked RFLP marker loci as those determined for Pl1 (resistance to race 1 in the line RHA266) on linkage group 1 of the consensus RFLP map of the cultivated sunflower. Pl1 and Pl6 thus appear either to be allelic or closely linked. The implications for sunflower breeding are discussed.
Keywords:bulked segregant analysis  Helianthus annuus  linkage  Plasmopara halstedii  resistance genes  RFLP  sunflower  downy mildew
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