甘薯茎尖培养主要影响因素研究

温度在２６～３４℃范围内，甘薯茎尖分生组织培养均可成苗。在此温度范围以外，因品种不同成苗率有所差别。培养适宜温度为２８～３０℃。２２～２４℃条件下，甘薯茎尖分生组织虽能生长，但不能成苗。培养基ｐＨ值４６～７０对较易培养成苗的甘薯品种成苗率无明显影响，但ｐＨ值过高对较难培养成苗的品种有影响。培养基琼脂浓度０４％～０７％均适于甘薯茎尖分生组织培养。

Studies on the Main Factors of Stem Tip Culture for Sweet Potato

Within the range of 26～34℃,seedlings could be formed from the generating tissues of sweet potato stem tip in vitro. Beyond this range of temperature, the seedling rate was different with different varieties. The optimum temperature was 29±1℃. Although generating tissues of stem tips could grow, seedlings couldn't be formed at 22～24℃. Medium pH 4.6～7.0 had no obvious effect on seedling rate of easy germinating varieties, but high pH value had an effect on hard germinating ones. The concentration of medium agar 0.4%～0.7% was suitable for generating tissue culture of stem tip for sweet potato.