日光温室和露地甜樱桃红艳秋季枝叶中氮代谢的比较

以日光温室和露地栽培的6年生槽栽甜樱桃红艳植株为试材,对秋季枝叶中全氮、总氨基酸、可溶性蛋白质、谷丙转氨酶、谷氨酰胺合成酶、硝酸还原酶的变化动态进行了测定。结果表明,温室条件下叶片中的氮向枝中运转的量少于露地;长枝枝皮、短枝中的可溶性蛋白质减少,而长枝木质中可溶性蛋白质增加很多;叶中可溶性蛋白质高于露地,而且叶中的可溶性蛋白质在整个秋季都维持在相当高的水平。秋季枝中总氨基酸的变化趋势比较复杂,而叶中总氨基酸的变化趋势则很有规律,都有增加的趋势。秋季叶中谷丙转氨酶、谷氨酰胺合成酶、硝酸还原酶的活性在整个秋季都没有急剧减少,有时还有增加,这表明叶片中的氮代谢一直在进行,并没有随叶片的衰老而减弱。

Comparison of nitrogen metabolism in shoot and leaf of sweet cherry tree in greenhouse and in field in autumn

The 6-year-old trees of Hongyan sweet cherry cultivar was used as material for studying the contents of total nitrogen, amino acid, soluble protein and the activities of GPT, GS, nitrate reductase in greenhouse and in field in autumn. The results indicated that the quantity of nitrogen transporting from leaf to branch in solar greenhouse was less than that in field. The content of soluble protein in long shoot bark and short shoot in greenhouse decreased, but it increased a lot in long shoot xylem. The content of soluble protein in leaves maintained in high level. The variable tendency of amino acid in branches was more complex and no regularity, but the tendency of amino acid content in leaves had increasing tendency. The activity of glutamate-pyruvate transaminase, glutamine synthetase, nitrate reductase in leaves didn't decrease sharply, and sometimes they were increased. This suggests that nitrogen metabolism takes place in all autumn and doesn't weaken with the senescence of leaf.