| 牛乳腺上皮细胞的分离培养 |
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| 引用本文： | 李吉霞,葛秀国,王文秀,张涌. 牛乳腺上皮细胞的分离培养[J]. 黑龙江畜牧兽医, 2012, 0(7): 4-6,174 |
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| 作者姓名： | 李吉霞 葛秀国 王文秀 张涌 |
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| 作者单位： | 天津农学院动物科学系;山东省滨州畜牧兽医研究院;西北农林科技大学动物医学院 |
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| 基金项目： | 国家高技术研究发展计划项目(2008AA101006);天津市高等学校科技发展基金计划项目(20100619) |
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| 摘 要： | 为了对牛乳腺上皮细胞(MECs)进行分离、培养和鉴定,并研究细胞分泌功能,试验通过胶原酶消化法分离得到了牛乳腺上皮细胞,采用传代法对细胞进行纯化,对细胞标志蛋白进行免疫荧光染色鉴定,通过体外诱导和RT-PCR分析鉴定细胞的分泌功能。结果表明:分离到的牛乳腺上皮细胞具有典型乳腺上皮细胞的形态特征,表达广谱角蛋白,经诱导后可分泌β-酪蛋白。
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| 关 键 词： | 牛乳腺上皮细胞 分离 培养 鉴定 |
Isolation and culture of bovine mammary epithelial cells |
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| LI Ji-xia,GE Xiu-guo,WANG Wen-xiu,ZHANG Yong. Isolation and culture of bovine mammary epithelial cells[J]. Heilongjiang Animal Science And veterinary Medicine, 2012, 0(7): 4-6,174 |
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| Authors: | LI Ji-xia GE Xiu-guo WANG Wen-xiu ZHANG Yong |
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| Affiliation: | 1.Department of Animal Science,Tianjin Agricultural University,Tianjin 300384,China;2.Shandong Binzhou Animal Science& Veterinary Medicine Institute,Binzhou 256600,China;3.College of Animal Veterinary Science,Northwest A&F University,Yangling 712100,China) |
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| Abstract: | To isolate,culture and identificatify the bovine mammary epithelial cells(MECs),studying the secrete function of the cells.The bovine mammary epithelial cells isolated with collagenase Ⅲ were purified using the passage methods.The cell marker proteins were determined with immunofluorescence staining,and the secrete function of MECs was identified by RT-PCR and in vitro induction.The results showed that the isolated bovine MECs had the typical morphological features,expressed a broad-spectrum of keratins,and secreted β-casein after induction. |
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| Keywords: | bovine mammary epithelial cells isolation culture identification |
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